蒙古羊全基因組測(cè)序及基于轉(zhuǎn)錄組分析的抗寒性狀遺傳基礎(chǔ)研究.pdf

1、內(nèi)蒙古農(nóng)業(yè)大學(xué)研究生學(xué)位論文獨(dú)創(chuàng)聲明本人申明所呈交的學(xué)位論文是我本人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。據(jù)我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包括其他人已經(jīng)發(fā)表或撰寫過的研究成果，也不包括為獲得我?；蚱渌逃龣C(jī)構(gòu)的學(xué)位或證書而使用過的材料，與我一同工作的同志對(duì)本研究所做的任何貢獻(xiàn)均已在論文中作了明確的說明并表示謝意。申請(qǐng)學(xué)位論文與資料若有不實(shí)之處，本人承擔(dān)一切相關(guān)責(zé)任。論文作者簽名：4交叢叢日期：’盤壘!塑=(!

2、望內(nèi)蒙古農(nóng)業(yè)大學(xué)研究生學(xué)位論文版權(quán)使用授權(quán)書本人完全了解內(nèi)蒙古農(nóng)業(yè)大學(xué)有關(guān)保護(hù)知識(shí)產(chǎn)權(quán)的規(guī)定，即：研究生在攻讀學(xué)位期間論文工作的知識(shí)產(chǎn)權(quán)單位屬內(nèi)蒙古農(nóng)業(yè)大學(xué)。本人保證畢業(yè)離校后，發(fā)表論文或使用論文工作成果時(shí)署名單位為內(nèi)蒙古農(nóng)業(yè)大學(xué)，且導(dǎo)師為通訊作者，通訊作者單位亦署名為內(nèi)蒙古農(nóng)業(yè)大學(xué)。學(xué)校有權(quán)保留并向國(guó)家有關(guān)部門或機(jī)構(gòu)送交論文的復(fù)印件和電子文檔，允許論文被查閱和借閱。學(xué)?？梢怨紝W(xué)位論文的全部或部分內(nèi)容(保密內(nèi)容除外)，采用影印、縮印或

3、其他手段保存論文。論文作者簽名：《蠡毯盤厶一指導(dǎo)教師馘：2駐!奴日期：兇墼么!!oPreliminaryAssemblyofMongolianSheepGenomeandTranscriptomeAnalysesofcoldtolenceinMongoliansheepAbstractMongolianSheep，beingknowntOitshardinessandgoodmeatqualityistheancientsheepbre

4、edinChinaThegenomecontainsagreatdealofpreciousgeneticresourcesInthisstudywesequenedthewholegenomeoftheMongoliansheepusingIlluminaHiseq2000sequencingtechnologyplatformandthewhole—genomeshotgunsequencingapproach，inordertop

5、rovidethebaseoffilteringthegeneswithfinepropertiessuchasanticoldnessdiseaseresistanceere。Theresultshowedthatthegenomesizewasestimatedin29lGbAssemblycontigN50lengthiS201KbseaabldN50l朋gthiS27313KbAverageGC％ofthesequencewas

6、42％，andtheGCaveragedepthiSmoTethan40incoveragedistributionRepeatsequcnceisI0lGbaccountingfor3471％andtransposableelementsiSaccountingfor90％inrepeatsequenceUsingthegenestructureandgenefunctionannotation，21，704geneswerepred

7、icted，thefunctionof20，600geneswerenoted，thesizeofthenon—codingRNAaccountsfor0OI5％ofthegenomeTherearel7，015genefamiliesinMongoliansheepBasedonthegenomeresearch，weusedaCGHsystemtoidentifytheCNVsinthesheepgenomeandconstruct

8、edtheCNVsmapjnMongoliansheep，Kazakhsheep，Tibetansheep，HusheepandDorpersheep5lCNVRsweretestedcontaining24gains，22lossesand5gain—lossesTheHBBgenewasamplifiedinMongoliansheepandTibetansheepwhichmaybeattributedtoadaptability

9、inlowoxygenandhighaltitudeenvironmentForthecold—resistaneeofMongoliansheep，DoperSheepasreference，wesequencedthetranscriptomeoffat，muscles，adrenalglandsandhypothalamusinthecoldconditionusinglllumina／Solexatechnologyandsel

10、ected529，75，268andl61differentlyexpressedgenesAccordingtodifferentlyexpressedgenesof伍ttissuebyKEGGsignalingpathwaytheresultsweremainlyrelatedwithfatcelldifferentiation，lipidsynthesis，energymetabolismI33up—regulatedgenesw

11、erefilteredinfat，inwhichRXR，F(xiàn)ATP，AOP7involvedinPPARcellsignalingwiththefunctionofacceleratingpreadipocytedifferentiationandtransportinglong—chainfattyacid；GB氏T3ALDHinvolvedinglycerolipidmetabolismsignalingpathwayandregul

12、atethesynthesisoftriglyceride；1RSlandLEPinvolvedintheregulationofenergymetabolism；theribosome28Ssubunitandl8Ssubunitinvolvedinproteins審nthesis10differentlyexpressedgeneswhichplayedanimportantroleinlipidsynthesisandenergy

13、metabolismwereselectedtobeverifiedusingqPCRTheresultswereequivalenttORNA—seqIncoldconditions，theleveloflipidsynthesisandenergymetabolismandproteinsynthesisinfattissueofMongoliansheepwashigherthanDoperSheep，whichshowedMon