生防菌Bacillus cereus ANTI-8098A對青枯雷爾氏菌微生態(tài)致弱機理的研究.pdf

1、福建農(nóng)林大學博士學位論文生防菌BacilluscereusANTI8098A對青枯雷爾氏菌微生態(tài)致弱機理的研究姓名：林營志申請學位級別：博士專業(yè)：農(nóng)藥學指導教師：尤民生2003.5.1AbstractThebacterialwiltcausedbyRa蠡toniasolanacearumisoneofthemostimportantdiseasesinthecropThemicroecologicalattentuationoftheR

2、solanacearumcausedbythebiocontr01agentBacilluscereusANTI8098AwasstudiedinthisPapeLincludingthedifferentiationofpathogenicityofRsolanacearumanditsdistributionintheplant，physiologicalandbiochemistrycharacteristicsofANTI809

3、8AanditsfermentationcharacteristicsandtechniquethemicroecologicalattentuationofRsolanacearumcausedbytheselectionofbiocontrolagentbetweenvirulentandavirulentRsolanacearumpol：’ulationsundertheditierentconditions，andthebioc

4、ontroIeffectsofmieroecologicalaRenuafiontothebacterialwiltdiseasePathogenicitytesttoalargeamountofRsolanacearumstrainscollectedfromthefieldshowedthattheobviousdifferentiationofpathogenicityexistedamongthem，eveninthediffb

5、rentstrainsfromthesameplantStudiesonthedistributionofRsolanacearumininfectedplantsatdifferentstagesindicatedthattheconcentrationofpathogenicbacteriumdecreasedwiththeincreaseoftheplantheightInadditionRsolanacearumwasfound

6、inthehealthypeanut，whichcouldexistandreproduceintheplantbuttmabletocausethediseasesymptomthestablevimlentandavirulentstrainswereobtainedasthestandardstrainsforthefollowingexperimentsthroughisolationpurificationandseveral

7、timesofsubcultureTheconditionsoffermentativecultureforbiocontrolstrainsBacilluscereusANTI8098AwerestudiedTheresultsindicatedthat：theoptimumconditionsforbacteriaIgrowthwerein250mLflaskwith25mLmediumofpH75shakingatthespeed

8、of200rpmat28士1℃TheconcentrationofsporewasuDt0182X105cfo／mlat36hafterinoculationof10％liquidcultureunderthisconditionToimprovethelevelofANTI8098Afermentationacontrolsystemwasdesignedandconstructedtocontrolfermentationparam

9、eterssuchastemperature，rotatingspeed，DOandpHTheprogramcontrolcouldbesettochangefermentativeparametersatdifferentstagesoffermentationaccordingtotbesimulativesetupschemeAtelecontrolsubsystembasedonTCP／IPnetworkwasdeveloped

10、toexecutetheremotecontrolofthefermentationsysternWhenthefermentationisdonein50Lfermenterunderthecontrolofthissystern，theconcentrationof5762106cfu／m1couldbeachievedin40hoursThesignificantdifferenceswerefoundintheinhibitio

11、ntestforthebacterial—wiltdiseasebiocontrolagentANTI8098AtovirulentandavirulentstrainsofRsolanacearumThe50％lethalconcentrationofANTI8098AtovirulentstrainofRsolanacearumwas124％。333％lowerthanthattotheavirulentstrainTheinhib

12、itionrateofANTI一8098Atothevirulentstrainwas0158406％higherthallthattotheavimlentstrainTheinhibitiondiffereneesofthestrainANTI一8098Atothevirulentandavimlentstrainsresultedintheattenuationbymicroecologicalselectionthatkille