25821.干旱脅迫條件下發(fā)菜的差異蛋白質(zhì)組學(xué)分析

1、寧夏大學(xué)碩士學(xué)位論文干旱脅迫條件下發(fā)菜的差異蛋白質(zhì)組學(xué)分析姓名：王星申請學(xué)位級別：碩士專業(yè)：植物學(xué)指導(dǎo)教師：王俊20100301AbstractNostocflagelliformeisakindofterrestrialnitrogenfixingcyanobacteriaithasimportanteconomicandecologicalvalue，andit’Sthefirst—gradeStateprotectionThisp

2、aperresearchedtheproteomechangesofNflagelliformeindifferentdroughtstressconditionsbyproteomicsmethodsandtechniques，identifiedthedifferentproteinsrelatedtodroughtresistanceandexplaineditsbiologicalfunctionTheresultslayedf

3、oundationforthedroughtresistantmechanismof^7=flagelliformeAtthe戤tinetimethepaperstudiedthephysiologicalchanges(suchasosmoticadjustmentsubstancesandprotectiveenzymeactivity)otMflagelliformeunderdifferentdroughtstressThema

4、inresultsofthisstudyareasfollows：1TheresearchdeterminedthecontentchangesofProandMDAofNflagelliformeunderdifferentdroughtstress，aswellastheSOD，POD，CATactivitychangesweremeaslnedtheresultsshowedthat：Witllthedroughtstresshe

5、avierthecontentofProandMDAincreasedSODandPODactivityincreased謝tlldroughtstressfnstandthendecreased，CATactivitydecreasedwiththedroughtstresssignificantlyincreased；2Inordertoestablishatwodimensionalgelelectrophoresis(2DE)p

6、rotocolforproteomicstudyofNflagelliforme，weoptimizedextractingmethodoftotalprotein，lysismethod，loadingquantityandthekeyprocessofIEFandSDSPAGETheresultsshowedthattheproteinspotsweredistributedmainlywithintherangeofpH4~7Im

7、provedTCAmethodwasobviouslyimprovetheproteincontentandresolutionpatterns1ysisbuffercontained60mmol／LD耵andloadingquantityofsampleproteinwere15mg／(24clnIPGtrip)couldobtainclearmapandbetterseparationeffectMorethan800protein

8、spotswimpH47weredetected3Inthisresearch，twodimensionalgelelectrophoresis(2DE)andbiomassspectrometrywereappliedtesearchdifferentialexpressionofproteimofⅣflagelliformeindifferentdroughtStressconditions，n圮resultsshowedthat8

9、19proteinspotsweredetectedwhenthemoisturecontentofMflagelliformeis661％，724proteinspotsweredetectedwhenthemoisturecontentis423％，618proteinsspotsweredetectedwhenthemoisturecontentis881％，769proteinspotsweredetectedwhenthemo

10、isturecontentis69％31proteinsspotsshowmorethan2foldchangesinabundancedifferentiallyexpressedweredetectedbyImageMaster2DsoftwareAmongtheseproteinspots，4wereupregulated，20weredownregulatedand7wereundulatoryregulated24differ

11、entialproteinspotswereidentifiedbybothMALDITOFTOF／MSandproteindatabasesearchingTheyweresecretedandsurfaceproteincontainingfasciclinlikerepeats(Spot2，7)、septumassociatedprotein(Spot5)、Cphycocyanin—lbetachain)(Spot6)、Perox

12、iredoxin)(Spot8，11)；Mncontainingcatalase(Spot10)、superoxidedismutase(Spot20，26)、DNAbindingferritinlikeprotein(Spot22，23，24)、Glutaminesynthetase(Spot14)、Fmctose／tagatosebisphosphatealdolase(Spot17，18)、Galactosemutarotasea

13、ndrelatedenzymes(Spot19)、PredictedmembraneGTPaseinvolvedinstressresponse(Spot15)、DalanineD—alanineligaseandrelatedATPgraspenzymes(Spot16)，andtherearefiveunknownproteinsThesedifferentialproteinsmainlyinvolvedinstructuralp