25587.基于硅玻基底材料的細(xì)胞電融合芯片的仿真及實驗研究

1、重慶大學(xué)碩士學(xué)位論文基于硅玻基底材料的細(xì)胞電融合芯片的仿真及實驗研究姓名：夏斌申請學(xué)位級別：碩士專業(yè)：生物醫(yī)學(xué)工程指導(dǎo)教師：鄭小林201004重慶大學(xué)碩士學(xué)位論文英文摘要IIABSTRACTCellfusiontechnologyisalsocalledcellhybridizationinwhichtwomecells(protoplasts)arecontactedonebyonethentheirmembranecytoplasm

2、nucleararefusedbyusingsomebiologicalchemicalphysicalmethods.Thehybridmayobtainsgeicmaterialfromtwomeparentcells.Thusitachievesnewbiologicalgeicacteristicsmaybeusedtodevelopnewspeciesstraincellengineeringproducts.Thedevel

3、opmentofcellfusiontechnologyhasgonethroughthreestages:biologicalchemicalphysicalinducementinthesecellelectrofusionwasdevelopedasanewphysicalmediatedwayfcellfusion.Comparedtotraditionalcellfusionmethodscellelectrofusionte

4、chnologyhasmanyadvantagessuchaseasyoperationhighefficiencyharmlesstocellssuitablefinstrumentationapplicationssoon.Howevertherearestillsomeproblemsinthetraditionalcellelectrofusionmethodincludingdifficulttoobservinghighvo

5、ltagerequiredwhichhindereditswideruse.Indertosolvetheseproblemsanewcellelectrofusiontechnologybasedonchiphasbeensetup.Inourgrouprelatedstudyhasalsobeencarriedoutseveralkindsofcellelectrofusionchipshasbeendevelopedwhichco

6、uldbeusedfcellfusionunderlowvoltagedriven.Accdingtothepreviousstudiesanewchipbasedonsiliconglasssubstratesisdevelopedinourgroupindertofurtherimprovethechiparchitectureachievebetterfusioneffect.Thischipcannotonlyreducethe

7、drivevoltageimprovefusionefficiencybutalsocanbeusedunderaninvertedfluescencemicroscopetoobservethefusionoffluescencelabeledcells.InthisthesisfirstthesimulationoftheelectricfieldwithinbothsiliconglassbasedchipSOIbasedchip

8、isbedone.Thesimulationresultsshowthatusingthesiliconglassbasedchipcangreatlyreducedrivenvoltagethesamesizeofthemicrochannelelectrodesstructuredesignmakestheelectricfielddistributemeunifmwhichcaneffectivelyimprovethecellf

9、usionefficiency.Thesimulationresultsprovideastrongtheeticalsuppttochipfabricationpackaging.ThenthepackagedchipisusedtodosomealignmentfusionexperimentsonHEK293NIH3T3cells.Experimentalsimulationresultsarematchedeachotherus

10、ingthedrivenvoltageunder10Vcouldinducecellalignmentfusionwhichismuchlowerthanusingpreviouschipstheunifmelectrodesstructurealsoimprovesthefusionefficiency.OtherwisetheexperimentonheterologouscellalignmentfusionofHEK293NIH