昆明山海棠殺蟲活性成分研究.pdf

1、西北農林科技大學碩士學位論文昆明山海棠殺蟲活性成分研究姓名：師寶君申請學位級別：碩士專業(yè)：農藥學指導教師：吳文君20040601AbstractAnavailablepathwaytodevelopnewclassesinsecticidesistofindnewleadmoleculesfromplants.ForthispurposeInsecticidalcomponentsofTripterygiumhypoglaucumwer

2、estudiedinthispaper.FourinsecticidealconstituentsofTripterygiumhypoglaucumwereisolatedbymeansofphytochemistrycombinedwithbioassaymethod.ThestructuresofthecompoundsweredeterminedbyHNMR“CNMRHHCOSYHMBCHMQCandmassspectralana

3、lysisaswilfortrine(sb01)wilforgine(sb02)wilfordine(sb03)andwilforine(sb04).bioassayshowedsb01andsb02hadonlystomachictoxicityagainstthe4“l(fā)arvaeofMythimnaseparata，butsb03andsb04hadstrongtopicaltoxicityagainstthe4“l(fā)arvaeofM

4、ythimnaseparate..Mediumnarcosisdose(NDso)ofsb03andsb04were0.0033uglarvae10.0006uglarvae1.Duringthecourseofbioassaywithfourinsecticidalconstituentsagainstthe4slarvaeof場thimnaseparatawefounddiferenttoxicsymptoms.Whenwilfor

5、dineandwilforinetreatedonthenotumofD力thimnaseparatethelarvaeswerenarcoticafter2hoursrevivedcompletelyafter8hoursthennarcoticagainafter24hourspartlarvaesweredeadafter48hours.Thelarvaesfeedthewheatleavesofsb01andsb02werena

6、rcoticafter8hoursrevivedcompletelyafter24hours.Theinsecticidalscopeofwilfordineandwilforinewerestudied.ResultsshowedthatwilfordineandwilforinehadtopicaltoxicityagainstMythimnaseparataMamestrabrassicaePlutellaxyllostellaM