鬼臼多糖分離提取及免疫藥理作用研究.pdf

1、甘肅農業(yè)大學碩士學位論文鬼臼多糖分離提取及免疫藥理作用研究姓名：帥學宏申請學位級別：碩士專業(yè)：基礎獸醫(yī)學指導教師：葉得河胡庭俊20060601SUA謹MARyItiswidelyrecognizedfl】atPodophyttumplaysmultipleroles汝pharmacologicalandbiologicalfunctionasatraditionalChineseherbalmedicineThispaperisperf

2、ormedOUextractiontechnology，pmificationandimmunefunctionofPodophytlumEmodipolysaccharides(PEP)強emainresultsareasfollows：1ExtractiontechnologyandcomponentsofPEP5009Podophyllumwasdegreased、eliminatedpigmentafterbeingdesicc

3、atedpowderofPodophyllumwasextractedforhoursinhotwateraccordingtodesignedsolidliquidratio，temperature，time，Then，liquidwasdealt謝氌ethanol、acetone、aetherrespectivelyFinally，3199ofPodophyllumEmodipolysaccharideswithoutamylurn

4、orproteinwasobtainedThepolysaccharidesespecialapiceswereseenbytheinfraredspectrumscan：3412，47cm“1，2928。50era“，164825cm“1，156028cm“1，1368。97～t45681crn5，92970cm～and85037era’：魏wasprovedbyThinlayerchromatogramsthatPEPwascomp

5、osedofglucose，fructose，mannoseandarabinose2Funeti好拄ofimmuneadjustmentTheinvestigationsonimmurlefianctionsofPEPmainlyinclude：spleenindex，thyrunsindex，contentofbacteriolysininserum，lymphocytesproliferation，geneexpressionan

6、dtheeffectonreactiveoxygenspecies(ROS)freeradicel21EffectofPEPOnspleenindex，thymusindexandcontentofbaeteriolysininsernnlOnDay1、3、5、7、PEP(200mg／kg)treatedtomousebyigcanimprovethespleenindex。difference(PO05)comparedwithcon

7、trolw8sobservedButtilepolysaccharidehasnoeffectonthethymusindexorthecontentofbacteriolysiainscramOnDay1～7，PEP(200mg／kg)treatedtomousebyipCallimprovespleenindexobviously。thereweresignificantdifference(PO01)comparedwithcon

8、tr012。2EffectofPEPOillymphocytesproliferationThelymphocytesisolatedfromroutinespleenwereculturedaccordingtodifferentconcentrationsofPERAfter3days，MTTmethodwasusedtoinvestigatetheeffectofPEPonlymphocytesproliferation，ther

9、esultshowsedPEP髓differentdosehaddifferenteffectsOniymphocytesproliferation50gg／mlPEPhadobviouslyeffect，100pg／ml、20099／ml、40099／mlPEPhadmoreeffectPEPwasabletoinducethelymphocytesproliferationincooperationwi也ConA。ItalsoCan