Th1細(xì)胞因子的含量對羊胚胎移植受胎率的影響及中藥提高受胎率的機(jī)理研究.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文Th1細(xì)胞因子的含量對羊胚胎移植受胎率的影響及中藥提高受胎率的機(jī)理研究姓名：苗玉青申請學(xué)位級別：碩士專業(yè)：臨床獸醫(yī)學(xué)指導(dǎo)教師：李鐵拴20050612StudyonthecontentofThlcytoldnesaffectingfecundationrateofeweontheembryotransplantationandthemechanismofherbalmedicineincreasingfecunda

2、tionrateAbstractGraduateSmdem：MiaoYuqingMajor：ClinicalVeterinaryAdvisor：Liq]eshuanThisstudywasdesignedtodiscussthecorrelationbetweenthecontentof1111cytokinesandthefecundationrateontheembryotransplantation，researchthefunc

3、tionofnitricoxide(NO)intheembryoimplantationandgrowth，andexplainthemechanismofherbalmedicine“AnTaiSan”O(jiān)ilpromotingthefecundationrateInthisexperiment，basedonembryotransfertechnique，血eanthorscollectedplasmanduterinemilkfro

4、mdonorsandreceiptsatthetimeofembryotransplantingandthereceipts“plasmwascollectedaftertwoeStlOUScyclesThecontentofcytokineswasmeasuredbyELlSAandthecontentofNOwasmeasuredthroughthemethodofnitricdeoxidizeenzymeTheresultswer

5、ethatthecontentofThlcytokinessuchas11NF—a、狂7NnIL一2inplasmoruterineIIlilkofgoatsandsheepwhowereestrouswassignificantlyhigherthanthenormalpregnantgoatandsheep(PO05)11lisindicatedthatitsadverseforembryoimplantationandgrowth

6、tosecretedThlcytokinessuperfluouslyItwasimportantforembryogrowthaftertransplantationtokeepthereceipts、uterusimmuneconditionconformingtothedonorsnecontentof伯1cytokinesinplasmofpregnantgoataftertwoesllouscycleswaslowerthan

7、thatatthetimeofWansplantingButthedefferentwash’tsignificant(P005)ThesefindingsdemonstratedthatthecontentofThlcytokinesdecreasedwiththedevelopmentofpregnancywhichisgoodtopregnancyThecontentofNOinplasmofgoatandsheepwhowere

8、estrouswassignificantlyhigherthanthenormalpregnantgoatandsheepfP005)111isresultshowedsuperfluousNOmaydoharmtoembryos，andledtoearlierembryolossAtthetimeofembryotransplanting，thechangingtendencyofthecontentofNOwasconformed

9、ton1cytokinesinplasmofgoatreceiptsnisresultdemonstratedtherewasacorrelationbetweenNoandThlcytokines，F(xiàn)urthermorewefoundtheNOcontentofpregnantewesonpregnant40dayswassignificantlyhigherthanthetransfertime(PO05)，butwhichwass

10、ignificantlylowerthanestrousewesatthesametime(PO05)ThecontentofNOincreasedwiththedevelopmentofpregnantinordertomeettheneedsofembryogrowthAfterembryotransplanting，20goatreceiptswererandomlydividedintotwogroups，includinghe