黃瓜ZYMV-CH和PRSV-W抗性遺傳和分子標記的研究.pdf

1、蘭州大學碩士學位論文黃瓜ZYMVCH和PRSVW抗性遺傳和分子標記的研究姓名：羅建華申請學位級別：碩士專業(yè)：植物學指導教師：浦銅良張海英20050601Abstract：Cucumber(CucumissaHvusL)isamajorvegetablegrowninChina’Sprotectivegroundsmthesunlightgreenhousescucumberdiseaseshaveincurredseveredamage

2、stothisplantespeciallythosegrowninwinter，theseyearsSomeevenresultinnoharvestinlargeareasTheC11inesestrainofzucchiniyellowmosaicvims(ZYMVCH)，watermelonstrainofPapayaringspotvirus(PRSV—W)andwatermelonmosaicvirus(WMV)aremaj

3、ordiseases廿latcausesignificantdiseaselossesincucumberTherefore，seekingmarkerslinkedtothevirusresistancerelatedgenesiSalwaysonthespotlightoftheresearchofmolecularbreedingscientistsBasedOilaRILpopulation(No8EuropeanXQiuPan

4、g)diseaseevaluationsareidentifiedandDNAwereextractedAfterwardstheBulkedSegregantAnalysis(BSA)methodwereadoptedtoscreentheexceptionalprimers，WhichwereusedintheanalysisontheF6sampleswiththeAmplifiedFragmentLengthPolymorphi

5、sm(AFLP)technologyMarkersobtainedwiththeabovemethod，representingthevirusresistancelinkagewerethellprocessed、vitlltheJoinmap30softwareandwerelocatedi11thecucumbergeneticmaD，Themaiorresultsofthisresearchareasfollows：1111ef

6、requencydistributionincucumberRILpopulationforDItraitsinvolvingresistancetothePRSV—WWMVandZYM=vCHshowtwopeaksThisindicatesthatresistibilitytothesediseasesarecontrolledbymaingene，withsomeminorgenesdemonstratingmoderateeff

7、ectsThc1inkageanalysisresultsshowedthatZYMV—CH，PRSV—WandWMVarelinkedandthesequenceiSZYMV10cM—PRSV一22cMWMV2Twobandsshowingpolymorphism(182bpand180bp)amongtwoparents，F(xiàn)1，susceptiblegenebulkandresistancegenebulkamplifiedbyAF

8、LPprimerpairE—ACG，MCAGTwomarkersnamedE—AC(MⅥ一CAG一182andEACG／M—CAG180were1inkedtOtheZYMVCHresistancerelatedgeneThegeneticdistanceswere5cMand8cMrespectively3TheE—ACG／MCAG一180markerhasbeentransferredintotwoCO—dominantSCARma

9、rkersnamedSCAR3109andSCAR4一134wi也thegeneticdistancesof11cMand13cMrespectivelyTheresultshowedthatthistwoSCarmarkerscouldbeusefuIinmarker—assistedseleetionincucumberbreedingwithZYMVresistance4PrimerEAAG／M—CAGrepresentspoly

10、morphismbetweensusceptiblegenebulkandresistancegenebulkinPRSVThiSmarkerwassupposedtobelinkedtOthePRSV—WresistancerelatedgenebytestingButthegeneticdistancesisalittlelarger，calculatedas24cM5AUofthemarkerswerelocatedinthecu

11、cumbergeneticmap，whichwasalreadybuiItupinourlaboratoryTheyWOUldbeusefulnotonlyforselectingcucumbervirusresistancebreedv撕etiesbutalsoleadapossiblewaytoclonevirusresistancegenesKeyWords：CucurnberVirusresistance—relatedgene