纖維蛋白凝膠攜載血管內皮生長因子修復大鼠坐骨神經損傷.pdf

1、遵義醫(yī)學院碩士學位論文纖維蛋白凝膠攜載血管內皮生長因子修復大鼠坐骨神經損傷姓名：劉恒申請學位級別：碩士專業(yè)：外科學指導教師：曹瑞治20090501遵義陜學院碩十學何論文英文摘要ExperimentalStudyofRatSciaticNerveInjuryRepairedbyVEGFMixedwithFibrinGlueasaCarrierAbstract：0bjective：Toinvestigatetheeffectsonangio

2、genesisandfunctionaloutcomeofexogenousvascularendothelialgrowthfactor(VEGF)mixedwithfibringlue(FG)asacarrierrepairingratsciaticnerveinjuryMethods：TheratsciaticnerveinjurymodelwasmadebymodulatedAllen’Sweightdropmethod36wi

3、starratswererandomlyintoexperimentalgroup(VEGFmixedwithfibingluegroup)andcontrolgroup(genetransfectiongroup)，with18ineachMicrovascularcount(MVC)，sciaticnervefunctionindex(SFI)，motornerveconductionvelocity(MNCV)，grossappe

4、arance，lightmicroscopicobservationwereperformedat4，8，12weeksaftermedicationNervesectionalimageanalysiswasperformedat8andI2weeksaftermedicationtomeasurethemeandiameterofneuraxis，thicknessofmyelinsheathandtotalnumberofmyel

5、inatedfiberResults：TherewasstatisticaldifferenceinSFI，MVCVtheMVCofthenerveat48weeks(p005)comparedwitllthecontrolgroupTherewassignificantdeviationinthemeandiameterofneuraxis，thicknessofmyelinsheathandtotalnumberofmyelinat

6、edfiberat8weekaftermedicationcomparedwiththecontrolgroup(pO05)Conclusions：FibringlueCanbeusedasacarrierforVEGFtorepairinjuredsciaticnervesTheseresultsindicatelocallyVEGFmixedwithfibinglueCanexertangiogenesisintheinjureds

7、ciaticnerveareaandenhanceneurologicalfunctionrecoveryoftllesciaticnerveinjuredratsThereWasnosignificantdeviationcomparedwiththeadministrationofgenetransfectionKeywords：Sciaticnerveinjury；Fibringlue；Vascularendothelialgro