腫瘤壞死因子-α基因-308G-A、-863C-A多態(tài)性與甲狀腺相關性眼病的相關性研究.pdf

1、山西醫(yī)科大學碩士學位論文腫瘤壞死因子α基因308GA、863CA多態(tài)性與甲狀腺相關性眼病的相關性研究姓名：呂兆琴申請學位級別：碩士專業(yè)：眼科學指導教師：孫斌20080405山西醫(yī)科大學碩士學位論文AssociationofTNF—Qgene一308G／A、一863C／Apolymorphismwiththyroid=iatedphthalmqpath】thyroidassociatedoDhthalmoDathyAbstractobie

2、ctiveToinvestigatetheassociationoftumournecrosisfactora(TNF一叻genepolymorphismsatposition一308G／A、一863C／AinShanxiregionChinesewiththyroidassociatedophthalmopathy(TAO)andtoexplorethegeneticbackgroundofTAOMethodsTNF—agenepol

3、ymorphismsatposition308G／A、863C／Aweredeterminedbypolymerasechainreaction—restrictionfragmentlengthpolymorphism(PCRPFLP)methodin42patientswithTAO、38patientswithGravesdisease(GD)and50normalcontrolsApplySPSSl15statisticalan

4、alysissoftwaretoreadallelesandgenotypesofindividualsamples，andeachgroupallelefrequenciesandgenotypecompared、析mchisquaretestResults(1)TNF0【geneatposition308existsG，AthetwoallelesandGG、GA、AAthethreegenotypes；TNF0【geneatpos

5、ition863existsC，AthetwoallelesandCC、CA、AAthethreegenotypes(2)ThestatisticresultsofgenotypeandallelefrequenciesofTNF—ageneatposition308：O)TherewerenosignificantdifferencesinthealleleandgenotypefrequenciesofTNF—aatposition

6、一308betweengroupTAOandnormalcontrolgroup、betweengroupGDandnormalcontrolgroup、betweengroupTAOandgroupGD(P005)②Stratifiedbysex，therewerenosignificantdifferencesinthealleleandgenotypefrequenciesofTNF—aatposition一308(P005)(3

7、)ThestatisticresultsofgenotypeandallelefrequenciesofTNFageneatposition一863：①TheAallelefrequenciesingroupTAOweresignificantlyhigherthanthoseingroupGDorthenormalcontrolgroup儼O05)；ThereWasalsonostatisticalsignificancebetwee

8、ngroupGDandthenormalcontrolgroup(PO05)③Stratifiedbysex，thefrequenciesofAalleleinmaleTAOpatientswerehigherthanthoseingroupGDorthenormalcontrolgroup(P005)InneithermalenorfemalepatientsofgroupGDandnormalcontrolgroup，signifi