靶向VEGF基因的siRNA對HeLa細胞中VEGF表達和細胞增殖的影響.pdf

1、東北師范大學(xué)碩士學(xué)位論文靶向VEGF基因的siRNA對HeLa細胞中VEGF表達和細胞增殖的影響姓名：左丹申請學(xué)位級別：碩士專業(yè)：細胞生物學(xué)指導(dǎo)教師：王麗20090601AbstractAn百ogenesisisthenecessaryprocessoftumoroccurrenceanddevelopment，itiscloselyrelatedtotunlorgrowthandmetastasisAnumberofgrowthfac

2、torshavebeenidentifiedaspotentialpositiveregulatorsofangiogenesisAmongthem，vascularendothelialcellgrowthfactorWEGF)isknowntobeoneofthemostimportantinducersofangiogenesisVEGFisnotolnycallinvolveintumorangiogenesisdirectly

3、orindirectlytopromotetumorgrowthbutalsoCallstimulatetheproliferationoftumorcellsintheformofautocrineTherefore，VEGFhasallextremelyimportantpositioninthe0CCUlTenCe，develpomentandprognosisofmalignanttumorsDifferentstudiesde

4、monstratedthatincreasedlevelsofVEGFexpressionhavebeenfoundinmosthumantumors，suchaScervicalcancersuggestingthatitmightbeallattractivetargetfortherapeuticinterventionaimingatpreventingtumorsfromrecruitingthebloodsupplythat

5、theyneedtosurviveRNAmediatedinterferenceisaconservedgenesilencingmechanismthatrecognizesdsRNAasasignaltotriggerthesequencespecificdegradationofhomologousmRNAandposttranscriptionalgenesilencingasaresultRNAitechnologyprovi

6、desnewideasforhighlyspecificgenesilencingtherapyInthisstudyweselectedcervicalcancercelllineHeLacellswhichhasahighexpressionofVEGFassubjectsARcrtransfactingVEGFgene—targetedsmallinterferingRNAvectorsintoHeLacells，weestabl

7、ishedstableexpressionoftheVEGFsiRNAcelllinesThepurposeofthisexperimentistoinvestigatetheeffectsofsmallinterferingRNAtargetingvascularendothelialgrowthfactor(VEGF)onVEGFexpressionandcellproliferationofcervicalcarcinomaHeL

8、acellsTheresultssuggestedthatsiRNACaneffectivelyknockdowntheexpressionofVEGFandinhibitthecellproliferationincervicalcarcinomacellsItshowsthatVEGFplayaroleintheoccurrenceanddevelopmentofcervicalcarcinomaInhibitingtheexpre