果蠅ECP對長時記憶和蛋白質(zhì)合成的影響.pdf

1、東南大學碩士學位論文果蠅ECP對長時記憶和蛋白質(zhì)合成的影響姓名：管海蘭申請學位級別：碩士專業(yè)：生物化學與分子生物學指導(dǎo)教師：汪道涌20120623英文摘要TheeffectofECPonlongtermmemoryandproteinsynthesisinDrosophila【ABSTRACTlGRADUATESTUDENT：GuanHailanSUPERVISOR：AssociateProfessorWANGDaoyongTheKey

2、LaboratoryofDevelopmentalGenesandHumanDisease，InstituteofLifeSciencesThemolecularmechanismoflearningandmemoryhasbeenaperplexingquestionforneurobiologistsandpsychologists‘MemoryCanbedividedintoshorttermmemorymediumtermmem

3、oryandlong—termmemoryTheformationoflongtermmemoryrequiresproteinsynthesisThepreviousresultssuggestthatECPanovelribosomeassociatedprotein，playscriticalrolesinaxonguidanceinDrosophilacentralnervoussystembyinfluencingprotei

4、nsynthesisC0150，aPelementinsertionmutantofecp，exhibitsdefectinlongtermmemoryHoweveritremainsunclearwhethertheLTMdefectinC0150isdirectlyrelatedtoecpHere，wefindthattheLTMdefectofC0150couldbefullyrescuedbyECPoverexpressionI

5、naddition，anotherPelementinsertionmutant，20241，whichhassimilarinsertionsiteasC0150alsoshowsdefectsinLTMAndSOdoeselavgal4ecp—RNAifliesTheaboveresultsundoubtedlysupporttheinvolvementofecpinLTMHoweverecp—RNAidrivenbymushroo

6、mbodyspecificgal4hasnoobviouseffectonLTMThissuggeststhatthelackofECPinMBdoesnotaffecttheLTMInperipheralnervoussysterm，thenumberofNMJ6／7boutondecreasessignificantlyinecpmutantsAlsodoesthethirdinstarlarvae’Sathleticability

7、TheelectrophysiologicalresultsrevealthatlackofecpdoesnotaffectthetransmissionoftheneurotransmitterEcpplayscriticalrolesonthedevelopmentofcentralandperipheralnervoussysteminDrosophilabuttheunderlyingmolecularmechanismrema

8、instobeclarifiedSeverallinesofevidencesupportthatecpmightbeinvolvedinproteinsynthesispathwayInordertoexploretheexactinvivoleofECPinproteinsynthesisinDrosophilaaninvivoGFPreportersystemWaSemployedTheresultsfromthissystems