The Effect of Micro-Rna 21 and Interleukin-6 on Development Ofheart Failure.pdf

1、Objective:we determined the overall effects of microRNA-21 and Interleukin-6 on development of heart failure by aggregating literature and subjecting studies meeting the inclusion and exclusion criteria to statistical me

2、ta-analysis.
　　Background:microRNAs are short regulatory RNA's that act as negative regulators of gene expression by inhibiting mRNA translation or by promoting mRNA degradation.Experimental evidence is rapidly accumu

3、lating,which demonstrates that microRNAs constitute a new mode of governing pathophysiological mechanisms in cardiac hypertrophy and heart failure.
　　Inflammatory activation with increased serum cytokine levels has be

4、en described as an important factor in the progression of the syndrome of chronic heart failure.In multifactorial analysis,elevated levels of interleukin-6 have been identified as the prognostic heart failure marker.

5、　　Materials and methods:original research papers were gathered from Tongji medical college library resources and online data bases.The papers were screened and coded;those meeting the inclusion and exclusion criterion we

6、re subjected to meta-analysis,using Cohen's d test to determine the effect size of each study.
　　Results:In microRNA-21 study six studies with 6 effect sizes met the stringent inclusion and exclusion criteria.The over

7、all mean effect of microRNA-21 from animal experiment on development of heart failure was highly significant (d=+1.36).The failsafe number associated with overall effect of microRNA-21 on development of heart failure in

8、animals was 76,a high number representing the number of additional studies in which microRNA-21 has negative or no effect on development of Heart failure required to negate the overall large effect size of+1.36.
　　In

9、interleukin-6 study seven studies with 7 effect sizes met the inclusion and exclusion criteria.The mean effect of interleukin-6 on development of heart failure from human studies was highly significant (d=+1.41) as well

10、as in animal experiments (d=+1.40).The failsafe number associated with overall effect of interleukin-6 on development of heart failure in human was 65 while in animal 26,a high number representing the number of additiona