生長抑素抑制人肝癌細胞株(HepG2)生長及對p16基因表達水平影響.pdf

1、山西醫(yī)科大學碩士學位論文生長抑素抑制人肝癌細胞株（HepG2）生長及對p16基因表達水平影響姓名：蘇俊俊申請學位級別：碩士專業(yè)：普通外科學指導教師：趙浩亮20100515山西醫(yī)科大學碩士學位論文TheEf代ctsofsomatostatinontheProliferationinHumanHepG2CellsandtheLeVelofGeneP16AbstractobjectiVeThrou曲usingmeFlowc”ometr)raI

2、ldReversetranscriptiontodeteminetlleH啪anHepG2Cellsw11ichis仃l：鋤edbysomatostatin，、ⅣestudyofsomatostatinaIlditsaIlaloguesontheh蚰1allHepG2CellsgrowtllmechanismandtheP16geneexpressionlevelsinordertoinVestigatetheinhibitionofh

3、epatomacellsorapoptosismechaIlismMethods111ecelllineHepG2werediVidedinto3groups：con仃Dl黟0upA，loWconcentrationgroupBandhi曲concentrationgroupcTheflowcytomet叫(FCM)w笛usedtodetectphaSedistributionofHlecyclesanddetecttheexpress

4、ionofp16genebyusingRTPcR。ResultsHumanHepG2Cellsa毹cted24hourSIaterbydi儂rentdens時SST，usingFCM，wec鋤discoVeredthattheG1cellcyclesofeachA罌Ioup、Bgroup、C黟oupoccupiedtheproportionrespectivelyis514l士327％、65426士341％、69019士375‰that

5、theScellcyclesis3686231％、20343土252％、18502土309％，thattheG2cellcycIesis1146士196％、1423l士383％、12479士318％。Comparetothecontr0IgroupA，lheG1ce玎cycleSofgroupBandgroupCwereincreaSedobViousJyButtheScellcycJesrelativereductionTheleVe

6、lsofPl6genewereexpressedineachgroupdetectedbyRTPCRbutBgroupandCgroupexpressedobViouslyConcIusions1Somatos_catinanditsaIlaloguesillllibittllemalignanth啪a11hepatomacellproliferation2SomatostatinaIlditsaIl址oguesinhibitmemal