高加索三葉草與白三葉草及其雜交胚離體培養(yǎng)技術(shù)的研究.pdf

1、內(nèi)蒙古農(nóng)業(yè)大學(xué)碩士學(xué)位論文高加索三葉草與白三葉草及其雜交胚離體培養(yǎng)技術(shù)的研究姓名：楊珍申請學(xué)位級別：碩士專業(yè)：生態(tài)學(xué)指導(dǎo)教師：王明玖何麗君20080501StudyonTissueCultureofCaucasianClover(TrifoliumambiguumBieb)andWhiteClover(丁repensL)andTheirHybridEmbryoAbstractClovers(TrifoliumL)aresomeannua

2、lorperenniallegumesthatcanimprovesoilfertility，increasegroundmulch，contralweedgrowing，keepsoilhumidityandreducesoilerosionCaucasiancloverhadthecharactersofhighyieldandstrongerresistancetOtheextremeclimate，buttheabilityof

3、nitrogenfixationwaspoorer；whilewhitecloverhadthecharactersofhighyieldandstrongerabilityofnitrogenfixation，butitsrootsystemswere。shallowandneedwetandwarmenvironmentInordertOcultivatethenewvarietyofcloveLsexploidCaucasianc

4、loverintroducedfromNewZealandandtetraploidwhiteclovergrewinHillDaxinganlinginInnerMongoliawereusedaSparentstOconductinterspecificdistanthybridizationCaucasiancloverandwhitecloverweretwospecieswhichhadgreatgeneticdifferen

5、tiationIntheprocess’ofhybridizatiofi，theproblemsofincompatibilityhybridembryoabortion，andhybridsterilityweremetTheseproblemscouldbeovercomeefficientlybythemeansofhybridembryotissueculturethenbackcrossOnthebasesofoldergen

6、erations’researchesforthecharactersofCaucasiancloverandwhitecloverthemeansoftissueculture，cytologyandisozymemakerwenusedtOstudythetechnologyoftissuecultureofhybridembryofromCaucasiancloverwhitecloverThemainresultswereasf

7、ollows：1TllekaryotypeformulaofCaucasiancloverWas2n=6x=48=44m4smbelongedto“2B”type；thekaryotypeformulaofwhitecloverWas2n=4x=32=22m10sm(2Sat)：belongedto“2A”type2TheregenerationabilityofstemsWashigherthanthatofleavesMS24DO2

8、5mg／L6BA05mg／L3％sucrose，MS2，4DO2mg／L6BA10rag／L2％sucrose，MS2，4DO25mg／L6BA10mg／LwerebetterforcallusinductionproliferationandregenerationofCaucaSianclover，andtherateofregenerationreached75％；thecompletedwhitecloverplantletWa

9、sobtainedontheMBmediumwith2，4D20mg／L6BAO75mg／L3WhentheCaucasiancloverWaSfemaleparent，whitecloveWaSmaleparent，pollinatedat8：009：00，andtheembryoWaspickedafter12～13dfrompollination，thehybridembryoWassterilizedbyNaCIO(10％)fo

10、r65～Tmin，thenculturedoneightimprovedMSmediathehighestgerminationrate(8％)couldbeobtained111ehigherfrequencypropagationWasgotonthemediaadded24D025mg／Land6BA050mg／L4ThestemsofhybridF1weretransplanted‘onmediawith6BA2mg／Land2