紫萁試管苗生產(chǎn)技術(shù)研究.pdf

1、Y808770華中農(nóng)業(yè)大學(xué)碩士論文紫萁試管苗生產(chǎn)技術(shù)研究StudyonProductiveTechniqueofOsmundaJaponicThunbTestTubeCultureSeedling研究生：孫紅緒Postgraduate：Sunhongxu指導(dǎo)教師：胡立勇教授Tutor：HuI；yongProtessor專業(yè)名稱：農(nóng)業(yè)推廣SpeciaIity：AgricuIturepopuIarjz。研究方向：蔬菜Researeharea

2、：vegetable華中農(nóng)、ik大學(xué)植物學(xué)院J|閣武漢：零零=：年f一，』C01)egeofplantHuazhongAgricultureUniversityWuhan，PRChinaNovember2003畢中教業(yè)人學(xué)學(xué)位論文：紫jt試管茁乍產(chǎn)技術(shù)例究StudyonProductiveTechniqueofOsmundaJaponicThunbTestTubeCultureSeedlingTutor：HuliyongProfesso

3、rPostgraduate：SunhongxuOsmundaJaponicqhunbisakindofpreciouspotherbmainlyforexportIocreatemoneyItsartificialplantinghasconsiderableeconomicresults，butitshardtofindtherootanditneedsalongperiodtocultivatetheseedlingMeanwhil

4、etherateofreproductionisverylowBasedonthesequestions，F(xiàn)rom2001to2003wemadestudiesofproductivetechniqueaboutOsraundaJaponicThunbseedlingcultivatedintesttube，usingplanttissueculturemediumAtfirst，weinoculatedtheOsraundaJapon

5、icThunbsporeandcultivateditonI／4MSculturebase，30dayslateraboutDO2cmsliceshapesubstancewasformed，AndthenwedrewDO5cmprothallium；carriedoutpropagationculture；andstudiedallkindsoffactorswhichmayaffectthereproductionoftheprot

6、hallium，theconditiontobreakupthesporpphyteseedlingthebuildingupseedlingtechniquesofsporpphytetcsttubeseedlingItshowsthatwiththelemperatureot、25。C，thesunshine12hrd“rthesunshineintensity20001x，andwiththeconditionofaddingsu

7、crose3091I，agaroagar69l。andPH58totheculturebase，thebetterculturebasesuitableforthcpropagationofprothalliumis1／2MSK7r50mg‘1。IBAl0mg1‘KH，04200mg1activecarbon0，5％Theprothalliumfittobreakupthesporpphyteseedlinghasthefeatureo

8、floosetexturesliceshapeandthickmeatqualityUndertheconditionofthetemperature20—28。C，humidity90％一100％，anawningoraforestwithfour—tenthsshadowandsix—tenthssunshine，youmaychoosematrixshreddedcoconutstuffingtobuildupseedlings，

9、theseedlingswhichhavemorethan3thickroots，3piecesofleavestheandlengthoftheleavesuptoover3cmcanhave976％survivalrate，ThisresearchformedasetofmaturequickreproductiontechniqueofOsmundaJaponicqhunbspore，thatistosayinoculatingO

10、smundaJaponicq／iunbspore，temptingtocultivateandformprothallum，andthencarryingoutpropagationculture，tcmptingittoformsporpphytcseedlingattherightmoment，eventuallyproducingOsmundaJaponicqhunbsporpphyteseedlingComparingwithH

11、eYifas‘WhreeSteps—cultureseedlingwithsoilUsingthetechniquetocultivateOsmundaJaponicq／／unbseedlingcanshortenatleast17monthstimetocultivateseedlingKeywords：Qf卅“，l如Japonicqliunb；spore；prothallium；testtubeseedling；buildupsee