球孢白僵菌侵染昆蟲(chóng)和附著胞形成時(shí)期受BbMPK1 MAPK調(diào)控的下游基因分離.pdf

1、IdentificationofthegenesregulatedbyBbMPKIMAPKinvolvedinthePenetrationofinsecteuticleinBeauveriabassianaDireetion:GeneEngineeringofMierobiologyMaj:MicrobiologyCandidate:ZhangJianqingAdvis:Prof.ZhangYon自u(píng)nBioteehnologyRese

2、arehCenter，SWUChongqing.P.R.ChinaJune，2009目錄中文摘要.........……，.…，.…，................................................................................……，....……IAbstraCt........................................................

3、……，...……，...........................................……111第一章文獻(xiàn)綜述.......................................................................................……，........……11.1昆蟲(chóng)病原真菌的侵染寄主過(guò)程.......................................

4、............................……11.2昆蟲(chóng)病原真菌的附著胞的形成.……，...........................................................……21.3MAPK信號(hào)途徑..........................................................................................……31.4

5、球抱白僵菌的MAPK信號(hào)傳導(dǎo)途徑.........................................................……61.5小結(jié)............................................……，...........................................................……6第二章引言.........................

6、.....................................................................................……92.1研究目的和意義.......................................……，............................................……92.2技術(shù)路線..........................

7、..........................................................................……10第三章球飽白僵菌侵染時(shí)期受BbMPKIMAPK調(diào)控的下游基因篩選...........……113.1材料.............................……，..........................................................

8、..............……113.1.1供試菌株......……，.....................................................................……H3.1.2供試?yán)ハx(chóng)......……，..............................................................................……113.1.3主要化學(xué)試劑.

9、.......................................................……113.1.4主要儀器設(shè)備..……，......................................，……113.1.5主要緩沖液配方........................................................................……lt3.1.6主要培養(yǎng)基....

10、.........................................……、....……，……，.....……123.2方法..……，..........……，........……、...............……、.，.....……，...............................……133，2、1菌株培養(yǎng)、..................................................

11、......……，.......................……133.2.2球抱白僵菌侵染菜青蟲(chóng)時(shí)期的樣品制備……，...............................……133.2.3球抱白僵菌侵染菜青蟲(chóng)時(shí)期RNA的提取及定量.……，................……133.2.4雙鏈cDNA的合成............................................................

12、..............……143.2.5抑制消減雜交..................................................................................……143.2.6cDNA片段回收二，..............................................，……143.2.7大腸桿菌感受態(tài)的制備........................

13、..........................................……143.2.8差示文庫(kù)構(gòu)建.................................................……，........……143.2.9差異表達(dá)基因篩選....................................……，...............................……巧3.2.10點(diǎn)雜交....

14、....................................................................................……巧3.2.11序列測(cè)定與分析……，.....................................................................……163.3結(jié)果與分析....................................

15、....................................................……163.3.1球抱白僵菌侵染時(shí)期的樣品制備、RNA的提取及檢測(cè)...............……163.3.2雙鏈cDNA的合成.....................................……173.3.3抑制消減雜交............................................……183.

16、3.4轉(zhuǎn)化大腸桿菌篩選陽(yáng)性克隆...............……，..................................……193.3.5差異表達(dá)基因篩選與測(cè)序結(jié)果分析...……，....................……，.......……193.4討論....................................................................................