東北雨蛙線粒體DNA遺傳多樣性的研究.pdf

1、編號(hào)：碩士學(xué)位論文碩士學(xué)位論文題目：東北雨蛙線粒體DNA遺傳多樣性的研究培養(yǎng)單位：生命科學(xué)學(xué)院專業(yè)名稱：分子生態(tài)學(xué)指導(dǎo)教師：董丙君副教授研究生：于佳琳完成時(shí)間：2017年4月沈陽師范大學(xué)研究生處制類別全日制研究生教育碩士同等學(xué)力IIGeicdiversityofthepopulationofHylajaponicabasedonmitochondrialDNAsequencesAbstractTheHylajaponicabelongs

2、toHylaHylidaeAmphibiamainlydistributedinChinasInnerMongoliaNtheastChinaabroadismainlydistributedintheKeanPeninsulaSouthKeaJapanFarEasternRussia.CurrentlyTherearefewstudiesaboutgeicofH.JaponicaInthedomesticStudiesmainlyre

3、latedtotheaspectsofphysiologybiochemistrycytochemistry.IntheoverseasThegeicdiversityofH.JaponicaH.suweonensisdistributedinKeanPeninsulawasanalyzedbyscholarofKean.Atotalof87sampleswerecollectedin14samplingsites(includingt

4、hedownloadhomologoussequencesintheGenBankoftheKeanpeninsula).TodiscussesthegeicdiversitydistributionsystemoftheH.Japonicapopulations.Themitochondrialcytbgenesequenceof845bpwasobtainedfromPCRamplificationsequencing.Thehal

5、otypediversityoftheH.japonicawas0824thenucleotidediversitywas000658TheseshowedthattherewasalowergeicdiversityamongthepopulationofH.japonica.Atotalof41variablesitesincytbsequencesfrominternalpopulationsdefined31haplotypes

6、amongwhichtherewere5haplotypessharedbetweenpopulations6haplotypessharedwithinpopulations20specifichaplotypes.Theconversionratioofbasetobasewas8.32.Therangeofgeicdistancesamonghaplotypeswas0~0.012themeangeicdistancewas0.0

7、08.OntheanalysisofstatisticalparsimonywkthedistributionofthehapotypesinpopulationsthebasisofphylogeictreeThetreewasdividedintotwobranchesonebranchwascomposedofhaplotypesinKeanPeninsulaanotherhadnogeographicaldistribution

8、.therewasnoobviousgeographicaldistributionofthewholepopulationseither.ThismaybecausedbytheacteristicsoftheH.japonicatheabsenceofacleargeographicalbarrier.Theadultpopulationshaveastrongdiffusionabilitysothewholepopulation

9、smaybemefrequentgeneexchanges.thereisnogeographicalisolation.Theterraininthentheasternregionisdominatedbyplainswithriversflowingwithoutmountains.MeoverthegeographicaldistancebetweenthentheastofChinatheKeanPeninsulaisrela