毛竹莖桿纖維細(xì)胞的發(fā)育生物學(xué)研究.pdf

1、南京林業(yè)大學(xué)博士學(xué)位論文毛竹莖桿纖維細(xì)胞的發(fā)育生物學(xué)研究姓名：甘小洪申請學(xué)位級別：博士專業(yè)：植物學(xué)指導(dǎo)教師：丁雨龍20050601StudyonthedevelopmentalbiologyoffiberinPhyllostachyseduliseulmsAbstractPhyllostachysedulis(Carr)HDeLehaie(Moso)hasthelargestdistributionareaandthehighestec

2、onomicalvalueinChinaItiswidelyusedformakingfurniture，constructionpulpandotherindustriesThestudyoftiberstructureisabasisforrevealingthemechanicalpropertiesofbambooByusingseveralmethodsandtechnologiesofcellandmolecularbiol

3、ogytheinitiationanddevelopmentoffiberinMosoculmwasfirstsystematicallystudiedInthiswork，theoriginanddevelopmentmodeoffiberwereelucidatedindetail，andthemechanismsoffiberwallformationandprogrammedcelldeathandthelong—livedch

4、aracteristicwerealsodiscussedThemainconclusionswereasfollows1FibersoriginatefromthesameprocambiumaSthevascularbundleelementslikevessels，sievetubesandparenchymacells，buttheirdifferentiationislaterThentheywouldundergocentr

5、ifugallydifferentiationanddevelopmentfromvascularbundleoutwards枷1thetibercapformedThedevelopmentalprocessoffiberscouldbedividedintothreecontinualstagessuchasfiberinitialfo刪tionandtheformationofprimarywallandsecondar了wall

6、Duringprimarywallformation，fiberunderwentsuccessivelyco—growthwithvascularbundleandintrusivegrowthWiththeformationofsecondarywallfiberwallunderwentcontinualthickeningwitllagingIntheformer4years，tiberwallhadadominantthick

7、eningeveryyearAfterwards，thedegreeofthickeningwoulddecreasegraduallyThethickeningregularityofsecondarywalldemonstratedthattheoptimalstageofcuttingforcultivatingMososhouldbeabout5yearsAtthemeantime，themultilamellatestruct

8、ureoftiberinalternatearrangementofnarrowandbroadlamellaeformedgradllallywhichcouldattributetotheregularchangeofclimateinayearduringsecondarywallformationlikethegrowthringofdicotyledonouswoodyplants2Theorganellesasmitocho

9、ndriaendoplasmicreticulumsandGolgibodies，andothersubstructuresliketransfervesicles，plasmalemmaandlomasome，andthecytoskeletalsystems，weretogetherinvolvedintiberprimarywallformationDuringtheprocess，ATPaselocalizedonfiberpl

10、asmalenunaplayedfunctionintheregulationofacidgrowth，whichcouldprovidethepreconditionsforprimarywallformationForasecondmessengerofenvironmentalstimuli，C礦couldinfluencefibernucleusandfacilitatedthesynthesisofRNAsandprotein

11、saboutprimarywallformationwhichcanupregulatetheformationoffiberprimarywallAlso，theacidphosphatase(AcPase)distributedontheplasmalemmaandtransfervesiclesandnucleusoffibercouldparticipateinthesynthesisandtransportationandex

12、ocytosisviavesiclesofnewcellwallmaterialsInaddition，theCa2ATPasepresentontheplasmalemmaandendomembranesystemsliketonoplastcouldplayrolesinthemaintenanceofCa2homeostesis，guaranteeingilaenormalphysiologicalmetabolismduring