濁點(diǎn)萃取火焰原子吸收鉛

1、1濁點(diǎn)萃取火焰原子吸收光譜法測(cè)定尿液中痕量鉛的研究楊雙王秀峰摘要:建立了以雙硫腙作為絡(luò)合劑，聚乙二醇辛基苯基醚(TritonX100)非離子表面活性劑為萃取劑的濁點(diǎn)萃取火焰原子吸收光譜法測(cè)定尿液中痕量鉛的方法。探討了絡(luò)合劑用量、平衡時(shí)間、平衡溫度、PH值、TritonX100用量及冰浴時(shí)間等因素對(duì)萃取效果的影響。該方法對(duì)鉛的檢出限為0.06μgL，相對(duì)標(biāo)準(zhǔn)偏差為2.93%(n=11)，回收率在96.7%～101.2%，線性范圍為0～0.

2、5ugml，最大富集倍數(shù)為40倍。用于尿液中痕量鉛的測(cè)定，結(jié)果令人滿意。關(guān)鍵詞：濁點(diǎn)萃取火焰原子吸收光譜法尿液鉛31前言...........................................................................................................................................41.1鉛的簡(jiǎn)介.............

3、............................................................................................................41.2尿鉛的簡(jiǎn)介.......................................................................................................

4、...............41.3濁點(diǎn)萃取的簡(jiǎn)介..............................................................................................................41.4火焰原子吸收光譜法的簡(jiǎn)介....................................................................

5、.....................61.5濁點(diǎn)萃取與火焰原子吸收光譜法聯(lián)用.........................................................................62實(shí)驗(yàn)部分....................................................................................................

6、...............................72.1儀器.................................................................................................................................72.2試劑................................................

7、..................................................................................72.3標(biāo)準(zhǔn)溶液的配制.............................................................................................................72.3.10.1gL鉛儲(chǔ)備液配制.

8、.............................................................................................72.3.21.0104molL的雙硫腙的丙酮溶液..................................................................82.3.3緩沖溶液的配制.......................

9、.............................................................................82.3.4TritonX100的配制...............................................................................................82.3.5硝酸甲醇溶液的配置..............

10、.............................................................................82.4樣品預(yù)處理.....................................................................................................................82.5濁點(diǎn)萃取.........

11、................................................................................................................82.6火焰原子吸收光譜法的測(cè)定.........................................................................................82.

12、6.1參數(shù)設(shè)置...............................................................................................................82.6.2測(cè)定步驟............................................................................................

13、...................92.6.3雙硫腙丙酮用量的影響......................................................................................92.6.4TritonX100的濃度對(duì)鉛萃取效率的影響.........................................................102.6.5pH值對(duì)鉛萃取

14、率的影響....................................................................................102.6.6緩沖液用量對(duì)鉛萃取效率的影響.....................................................................112.6.7平衡溫度對(duì)鉛萃取效率的影響......................

15、...................................................112.6.8加熱時(shí)間對(duì)鉛萃取效率的影響.........................................................................122.6.9冰浴時(shí)間對(duì)鉛萃取效率的影響........................................................

16、.................132.6.10黏度對(duì)鉛萃取效率的影響...............................................................................132.6.11共存離子的干擾..........................................................................................

17、.....133結(jié)果與討論.............................................................................................................................143.1檢出限、精密度的測(cè)定...................................................................

18、............................143.2最佳實(shí)驗(yàn)條件...............................................................................................................143.3分析特性.............................................................

19、..........................................................153.4樣品測(cè)定及回收實(shí)驗(yàn)...................................................................................................153.5結(jié)論..........................................

20、.....................................................................................154參考文獻(xiàn)................................................................................................................................