多功能復(fù)合微生物肥料的研制.pdf

1、江西農(nóng)業(yè)大學(xué)碩士學(xué)位論文多功能復(fù)合微生物肥料的研制姓名：鄭傳進(jìn)申請學(xué)位級別：碩士專業(yè)：土壤學(xué)指導(dǎo)教師：涂國全2003.6.1一一耄鯊塹墨壘燮皇塑墨翌塑里型墊鐾ABSTRACTTwostrainsofbacteriumBmucilaginosusandBmegateriumFarphosphaticumandastainofstreptomycesSjingyangensiswhichareusuallyusedtomakeintomic

2、robialfertilizerhavebeenstudiedinthispaperTheabilityof愛mucilaginosusteleaseminera】potassiumkhasbeentestedbymeansofinoculating&mucilaginosusintoKabsentmediumcontaining1％mineralpotassium。TheresultshowsthestrainofBmBDwhichi

3、sisolatedbyourlaboratoryhasstrongerKreleasingabilityTheKcantainedintheflaskandfermenterculturalliquidwhichhaveinoculatedBm—BDare474％and34％overtheCK秘1eP—discomposingabilltyof捏megateriumvarphosphoroushasalsobeentestedbymea

4、nsofinoculatingBmegateriumvarphosphorousintotheculturemediumwithCa3(P04)2orleithinastheonlyPsourceItWasfoundthattheavailablePwasgreatlyirmreased，whichthecontentofdissolublephosphorousintheculturalsolutionarerespectivly13

5、6and416timesovertheCK髓eresultofantimicrobialactivitytestingforSjingyangensisindicatesthatitpossessesbacteriostasficactivitytomanyspeciesof◇orGbacteriaandfungiWithaseriesoforthogonalexperimeritsandsinglefactorexperiments，

6、theoptimalnutrientmediumandthemostsuitablecultureconditionsforthethreefunctionalmicrobesareelicitedTheoptimalnutrientmediumfor黷mucitaginosusis05％ofcanesugar008％ofyeastabshact0Dj％of剁拽蔓S04008％ofMgSQ，0!％ofK2HPO，O，Ot％ofNaCIa

7、nd001％ofCaC03Themostsuitablecuitureconditionsforshakeflashculture丑mucilaginosusispH70，asuitablevolumeofliquidculturemediumin250mLflashis30mLthemostsuitableshakeffequenceis200r／min，themostsuitableculturetemperatureis30℃Th

8、atof艘megateriumParphosphorousis，O08％of(NH)2S04，1％ofglucose，O03％ofK2HP04，002％ofNaCI，002％ofKCl，O08％ofMgS04，0003％ofMnS04and0003％ofFeSO“Themostsuitablecultureconditionsforshakeflashculture丑megateriumparphosphorousisthatwitha

9、pHof70。avolumeofliquidculturemediumis30mL／250mL，frequenceis200r／min，culturetemperatureis34℃。FortheSjingyangensisthesuitablesolideculturaIconditionisapHof8，atemperatureof30℃，andawatercontentof25％Thenaccordingtotheresultst

10、hethreestainsofmicrobesaremassivelyculturedbymeansofliquidorsolidfermentation，TheirgrowthandmetabolismprincipleregularpatternalsobeenstudiedatonetimeThefinalfermentoffspringwhicharestilliving，arecollectedandmixedwi出文herm

11、aterialstomakeintobio—fertilizerThetestingresultsofthebiofertilizerindicateditmeasureuDtothecriterionofNY227一1994ofwhichthelivingmirobeismorethan500millionperkilogram，Thismeansthemanufactureprocesssummarizedafterlargenum

12、bersofexperimentsispracticableFinallytheeffectsandreasonsofthemicronalcompoundfcrtilizeronincreasingyieldofsomecropsandvegetablesarealsoStUdiedjnthepaperTheresultsshowthatthecompoundmicrobialfertilizerCallevidentlypromot

13、ecropstoabsorbPandK，andcarlpreventcropsframsomediseasesThussolvedtheproblemofcllrrentbio—fertilizershavingalleffectofslownessandsingularityKEYWORDS：CompoundMicroorganismF盯Ⅲi2erManufactureProcessEffectTestingfo，F(xiàn)inishedPr