甘藍(lán)型油菜矮稈突變體及矮稈基因分子標(biāo)記的研究.pdf

1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文甘藍(lán)型油菜矮稈突變體及矮稈基因分子標(biāo)記的研究姓名：黃天帶申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：吳江生20030601華中農(nóng)業(yè)大學(xué)2003屆碩士論文StudiesonaDwarfMutantandMolecularMarkersofDwarfGeneinBrassicanapus三AbstractThemaingermplasmusedinthisstudywasadwarfmutantnamedDs一1w

2、ithzhongshuangNO4(ZD4)andHuashuangNO3(HD3)ascontr01CharactersoftheyoungseedlingofDs1wereinvestigatedunderlightanddarkconditions，anditsothergrowinghabitsandagronomiccharacterswereidentifiedinfieldThereactionsofitshypocoty

3、landstemtodifferentphytohormonetreatmentswereobservedTheinheritanceofthegenefortheshortstemofDs1andtheallelicrelationshipwithanotherdwarfgermplasmDs2werestudiedRAPDtechniqueandcandidategeneapproachwereadaptedtodetectmole

4、cularmarkerslinkedtothedwarfgene(Dsl)Themainresultswereasfollows：1HypocotyllengthofDs一1grownunderlightconditionwasshorterthanthatofZD4，butnoobviousdifferenceunderdark；Ds1waslowersignificantlythanZD4inplantheightbranching

5、position，numberofelongatednodes，lengthofintemodeandmaininflorescence；butastothenumberoftotalnodesthereWasnosignificantdifferencebetweenthedwarfandthenormalvarietiesItshowedthatthedwarfphenotypewasMuetofewernumbersofelong

6、atednodes，shorterlengthofintemodeandmaininflorescence，lowerbranchingpositionsIncomparison謝tllthecontr01DsIhadfewersiliquesperplant，loweryieldperplantandhiger1000seedsweight，butnosignificantdifferenceexistedinbranchnumber

7、s，podsofmaininfloresenceandseedsperpod2Phytohormone6BA、BRhadnosignificanteffectstothehypocotyllengthofDs一1：L蛆andGA3enhancedhypocotylelongationsignificantlybutonly100mg／LGA3couldelongateitshypocotyltonormallength；6BA、IAA、

8、NAA、GA3、920、BRhadnoobviouseffectstothestemelongationofDs1，while600megLPP333reducedthestemlength，and600mg／LGA3couldpartiallyenhancethestemlengthreducedbyPP333butnottonormalTheseresultsindicatedthatthesignaltransductionfor

9、GA3inDs1wasnormal3GeneticanalysisshowedthattheplantheightsofF1populationwasintermediateoftwoparents，butpartialtodwarfparent，F(xiàn)1andRFlhadnosignificantdifferenceThesegregationratiooftallplants：shortplantsinF2populationaccor