兔圓小囊內(nèi)彌散神經(jīng)內(nèi)分泌細(xì)胞及免疫細(xì)胞發(fā)生發(fā)育研究.pdf

1、丫774897分類號密級’專目差≮犬等碩士學(xué)位論文單位代碼：10019學(xué)號：26035兔圓小囊內(nèi)彌散神經(jīng)內(nèi)分泌細(xì)胞及免疫細(xì)胞發(fā)生發(fā)育研究StudiesonDevelopmentoftheDiffuseNeuroendocrineSystemCellandtheImmuneCellinSacculusRotundusofRabbit研究生：指導(dǎo)教師：申請學(xué)位類別：專業(yè)領(lǐng)域名稱：研究方向：所在學(xué)院：型建塑金魚蓮數(shù)援獸醫(yī)硒主亟隨獸匡麴腿魚痤動

2、物匡生陵2005年3月AbstractThesaoculusrotundushavingvariousfunctionssuchasimmunityendocrineandassimilation，areonlyfoundinrabbitsInordertoelucidatedevelopmentofthediffuseneurocndocfine(DNES)andtherelatedimmunecellinsacculusrotund

3、usofrabbits，thestudies，usingthemethodsofimmunohistochemistryelecuonmicroscopicc”ochemistryandsoon，observedthedistributionandquantityofsubstanceP(sP)synaptophysin(SYP)，proteinS100，CD3frlymphocyte)，CD20(Blymphocyte)，CD57(N

4、Klymphocyte)andCD68(macrophage)inthesacculusrotundusof160thday’srabbitsMoreoverthesacculusrotundusareobservedbymeansofSEMin5thand15thdays’rabbitsHerefoliowstheresultsTheprogressiveincreasementofpositivecellswiththesubsta

5、nceP(sPLsynaptophysin(SYP)andproteinS100wasobservedinthelymphoidtissuesofsacculusmtundusofrabbitinthisstudyButtherewasvariousnessofemergencetimeandplaceinthesacculusrotundusMeanwhile，itcanbeensuredthatDNEScellsarerelated

6、tomaturityofthelymphoidcellsBasedonthehistochemistryobservation，thereappearstwotypesofcellinthelymphoidtissuesofsacculusrotundusinthe1stday：Atype(namedtypeIcell)showinglarge，pale，roundorovalshapes，haslargeandbrightnuclea

7、rbutlittlecytoplasmAgreatnumberofslenderprocesseswereobserved，andtheprotrusionsbetweenthecellsinterweaveeachotherformingloosereticulatedformatio。Therearemorethecellsintheearlydevelopmentofsacculusrotundus；Theothertypeofc

8、ells(namedtypeIIcell)aremostlyofdarkcolorandsmallsizeThenumberoftypelIcellsarelessthantypeIintheearlydevelopmentofsaceulusrotundusAtthe5thdaythereappearsomeroundlymphoblastswithlarge，darknucleusinthelymphoidtissuesofsacc

9、ulusrotundusThelymphocytesandmacrophagesincreaseprogressivelyThroughobservationandquantitativeanalysisfordifferentdays’sacculusrotundusoftheTlymphocyte(CD3)，Blymphocyte(CD20)，NKlymphocyte(CD57)andmacrophage(CD68)，itwasfo

10、undthatatthe1stdaythereappearanumberofCD68andCD57cellsotherthanCD3andCD20ceils；atthe10thdaythereappearfewCD3andCD20cells；asrabbitgrowingCD68、CD57、CD3andCD20cellsamallincreasedobviously；atthe40thdayCD3cellsandCD20cellsare

11、morethanCD68cellsandCD57cellsnotablyfP005)itisshowedthattherearecertaincorrelationsbetweenthesetypesofcellsbySPSS，UsingSEMmethod，itwasfoundthatthesaceulusrotundusofthe5thday’srabbitshavesmoothsurface，sparsevilluses，inthe

12、topofwhichthemareanumberofregularmicroforaminaInthe15thdaythevillusespresentashapeoffingerandhaveroughsurfaceinwhichtherearelessforaminaThesemorphologictransformmayberelatedtotheevolutionmechanismofnourishmentanddefensei

13、ninfantrabbitsTheanalyticalresultsaboveshowedthatthethreeindicatorsofDNEScellsappearindifierenttimeandplaceofthesacculusrotundusTheevidenceofrelatedimmunecellsimpliedthatemergencetimeofacquiredimmunitycellsislaterthannat