不同濃度臍血鉛對新生兒端粒長度、端粒結(jié)合蛋白TRF1及TRF2的影響.pdf

1、不同濃度臍血鉛對新生兒端粒長度、端粒結(jié)合蛋白TRFl及TRF2的影響摘要目的觀察不同濃度臍血鉛對新生兒端粒長度、端粒結(jié)合蛋白TRFl及TRF2的影Ⅱ向。方法收集青島市市立醫(yī)院2010年7月至2013年4月孕期無急慢性疾病、臍血鉛濃度100pg／L的足月順產(chǎn)兒臍血84例，應用原子吸收光譜法測定臍血鉛濃度。以臍血鉛濃度3099／L為分界分為極低水平臍血鉛組(臍血鉛濃度30“g／L)，低水平臍血鉛組(臍血鉛濃度30“g／L)。采用全血提取基因

2、組法提取臍血DNA，熒光定量PCR法測基因組DNA的端粒長度，ELISA法測端粒結(jié)合蛋白TRFl、TRF2的濃度。兩組問比較采用獨立樣本t檢驗，相關(guān)性采用Pearson相關(guān)性研究。結(jié)果①極低水平臍血鉛組端粒長度為06840540，低水平臍血鉛組端粒長度為0350_0285，兩組之問差異有統(tǒng)計學意義(t=2429，P=O020)；②極低水平臍血鉛組TRFl濃度為(pg／m1)772199431，低水平臍血鉛組TRFl濃度為90462173

3、46，兩組間差異有統(tǒng)計學意義(t=一3146，P=O003)；極低水平臍血鉛組TRF2濃度為(pg／m1)22029657042，低水平臍血鉛組TRF2濃度為18019428767，兩組間差異有統(tǒng)計學意義(t=2782，P=O008)；③端粒長度與臍血鉛呈負相關(guān)性r=O342，P=O027，即隨臍血鉛濃度的升高，新生兒端粒長度逐漸縮短；端粒結(jié)合蛋白TRFl與臍血鉛呈正相關(guān)性r=O482，P=O001，端粒結(jié)合蛋白TRF2與臍血鉛呈負相關(guān)

4、性r一0314，P=O043，即隨臍血鉛濃度的升高，端粒結(jié)合蛋白TRFl表達逐漸增多，端粒結(jié)合蛋白TRF2表達逐漸減少。結(jié)論新生兒端粒長度的變化與臍血鉛濃度水平相關(guān)，隨臍血鉛濃度升高，端粒長度逐漸縮短，端粒結(jié)合蛋白TRFl表達逐漸增多，端粒結(jié)合蛋白TRF2表達逐漸減少，即端粒的保護因子減少，有害因子增多。提示臍血鉛影響端粒長度通過改變端粒結(jié)合蛋白的表達來干擾端粒長度。碩士研究生：趙艷芳(兒科學)指導教師：馬海燕主任醫(yī)師Effectsof

5、differentumbilicalcordbloodleadlevelsontelomerelengthandtelomerebindingprotein(TRFl，TRF2)inneonateAbstractObjectiveToinvestigatetherelationshipamongumbilicalcordbloodleadlevelsontelomerelengthandtelomerebindingprotein(TR

6、F1，TRF2)inneonateMethodsWecollected84casesofumbilicalcordbloodfromterminfantswhosegestationwaswithoutacuteorchronicdiseasefromQingdaoMunicipalHospitalbetweenJulyof2010toAprilof2013andtheumbilicalcordbloodconcentrationwas

7、lessthan100“g／LWeusedatomicabsorptionspectrometrytodeterminetheconcentrationofumbilicalcordbloodleadandwedividedthe84casesintotwogroupsaccordingtotheleadlevelsofumbilicalcordbloodat30Pg／L，umbilicalcordbloodleadconcentrat

8、ionlessthan3099／Lwasverylowlevergroupandmorethan3099／LwaslowlovergroupToextractumbilicalcordbloodDNA，extractiongenomeDNAfromwholebloodwasusedFluorescencequantitativePCRwasusedtomeasurethetelomerelengthofgenomeDNAandELISA

9、tomeasuretheconcentrationoftelomerebindingproteinTRF1TRF2WeusedSPSS170toanalysethedifferenceoftwogroupsandtherelationshipwereanalyzedwithSpearmancorrelationresearchResult@Thetelomerelengthofverylowlevergroupwas06840540an

10、dlowlevergroupwasO3500285Thedifferencebetweentwogroupswasstatisticallysignificant(t=2429，P=0020)@TRF1concentrationofverylowlevergroupwas772199431(pg／m1)andlowlevergroupwas90462土17346(pg／m1)Thedifferencebetweentwogroupswa

11、sstatisticallysignificant(t=一3146，P=0003)TRF2concentrationofverylowlevergroupwas220296士57042(pg／m1)andlowlevergroupwas180194士28767(pg／m1)Thedifferencebetweentwogroupswasstatisticallysignificant(t=2782，P=O008)@Therelation