組胺和花生四烯酸體外誘導(dǎo)變態(tài)反應(yīng)性炎癥的發(fā)生及其干預(yù).pdf

1、第三軍醫(yī)大學(xué)碩士學(xué)位論文 1英文縮寫詞表 英文縮寫 英文全稱 中文譯名 A absorbance 吸光度 AA arachidonic acid 花生四烯酸 AI allergic inflammation 變態(tài)反應(yīng)性炎癥 bp base pa

2、ir 堿基對(duì) ddH2O double distillated water 雙蒸水 DEPC diethl pyrocarbonate 焦碳酸二乙酯 DNA deoxyribonucleic acid 脫氧核糖核酸 dNTPs deoxy

3、ribonucleoside triphosphates 脫氧核糖核苷三磷酸 EB ethidium bromide 溴化乙錠 EDTA ethylene dinitrilotetraacetic acid 乙二胺四乙酸 ELISA enzyme link

4、ed immunosorbent assay 酶聯(lián)免疫吸附試驗(yàn) H histamine 組胺 h hour 小時(shí) Ig immunoglobulin 免疫球蛋白 IL- 4 interleukin- 4 白介素- 4 IL- 5 interleukin- 5 白介素- 5 LTs leukotrienes 白三烯 MCP- 1

5、 monocyte chemotactic protein- 1 單核細(xì)胞趨化蛋白- 1 MCP- 3 monocyte chemotactic protein- 3 單核細(xì)胞趨化蛋白- 3 min minute 分鐘 mRNA messenger ribonucleic acid 信使核糖核酸 第三軍醫(yī)大學(xué)碩士學(xué)位論文 3The allergic inflammation in

6、duced by histamine and arachidonic acid in nasal polyps and skin in vitro and its interference Abstract Backgrand and Aims Allergic diseases are common in department of dermatology, and investigation of t

7、he mechanisms and development of new effective therapies are very important. It’s well proved that histamine and arachidonic acid are crucial mediators in this field. Research showed that nuclear factor-

8、 B(NF- B)would be activated after histamine bind to its specific H1 receptor, and then activated NF- B promoted the mRNA expression of many kinds of inflammation mediators. These events produced abundance in

9、flammation mediator proteins, which participated in and aggravated the inflammation. The anti-histamines can antagonise this phenomenon. In present study we cultivated nasal polyps and skin tissue in vitro

10、 and added histamine and arachidonic acid to observe whether they can induce the allergic inflammation. Several anti-histamines were added to detect if they can inhibit the inflammation. Materials an

11、d Methods The nasal polyps or skin tissue were cultivated with mizolastine, cetirzine, loratadine, fexofenadine, dexamethasone, and ciclosporin A separately, and then histamine and arachidonic acid were added. Twenty-fou

12、r hours later the total RNA of polyps or skin tissue was obtained and purified with Tripure isolation reagent. The mRNA expression levels of monocyte chemotactic protein- 1 MCP- 1monocyte chemotactic protein-3 MCP-3 regu

13、lated upon activation, normal T cell expressed and secreted RANTES eotaxin were analyzed by RT- PCR methods. The concentrations of interleukin- 4 (IL- 4) interleukin-5 (IL-5) and tumor necrosis factor- (TNF- ) in cultiva

14、ted supernatant were detected by ELISA methods. Furthermore the nasal polyps were cultivated with different concentration of mizolastine, and then histamine and arachidonic acid were added. We detect the MCP- 1 M

15、CP- 3RANTES and eotaxin by RT- PCR methods and the concentrations of IL- 4 IL- 5 and TNF-in cultivated supernatant were detected by ELISA methods.Results We cultivated the nasal polyps and skin tissue