長(zhǎng)春花總堿的開發(fā)及脫水長(zhǎng)春堿合成新工藝.pdf

1、大連理工大學(xué)碩士學(xué)位論文長(zhǎng)春花總堿的開發(fā)及脫水長(zhǎng)春堿合成新工藝姓名：丁亞芳申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化工指導(dǎo)教師：安利佳20050616長(zhǎng)春花總堿的開發(fā)及脫水長(zhǎng)春堿合成新工藝DevelopmentofVincaAlkaloidsandTheNovelSyntheticTechniqueofAnhydrovinblastineAbstractVincaisaIlimportantkindofmedicalplantbelongingt0

2、dogbanespeciesTherearemorethan100kindsofalkaloidsinvincaplantincludinganhydrovinblastine，vinblastine，vincfisdneandotherantitumormedicineusedinclinic，etcVincadrygrassandfreshglassasrawmaterialbyaseriesofmethods：aciddissol

3、vingalkaliprecipitationandextractionusingorganicsolvent，wegatheredandpurifiedvincaalkaloidsin011IexperimentsAddingacid，alkaloidscailbetransformedsaltandimprovedsolubilityinthesolutionSOalkaloidscarlbeinwaterphaseandbeene

4、xtractedfromvincaplant；ThenadjustingPHtobealkalinealkaloidscarlbeprecipitationinthewaterphaseExtractingusingorganicsolventthenremovingwaterphase，thuswecarlremovedothermixedsubstancewhichdissolvedinthewaterphaseNextadding

5、acidagainalkaloidsenterinwaterphaseagainAtthemomentaddingorganicsolventtowaterphasecanremovedoiledmixedsubstancesSOpurifiedalkaloidsremainedinthewaterphaseAdjustingPHofwaterphasetobealkalineoncemore，precipitationbecooled

6、anddriedintheVaCHtliTIuntilittobepowderThuswegainedroughvincaalkaloidsTherearemanykindsofalkaloidsinvincaalkaloidsAmongthesesubstances，thecontentofcatharanthineandvindolinearebiggerthevaluerespectivelyare12236％and490％Ino

7、urexperiment，catharanthineandvindolineasmaterials，F(xiàn)ejasoxidant，wefirstgainedaintermediathendeoxidizetheintermediausingNaBIhthusWeobtainedsynthesizedproductAt0“C，methanolsolutionwegainedcrystal，thatispurifiedanhydrovinbla

8、stineThecrystalyieldreached3429％AndthechemicalstructureofthecrystalisconfizmedbyUV，RMSand1HNMRComparedwiththemethodsinthedocuments，thenewsyntheticmethodispracticalandsuitableforlargescaleproductionOntheSalTIeconditionofl

9、abomtory，productofthenewmethodismo挖pure1essotherproductandtotalyieldishighestVincaalkaloidsasmaterial，byseveralsteps：addingFej，NH3H20，extractionsolution(chloroform：methanol=1：1)，NaBFh，wegainedacertainsubstancewhichisnear

10、lypureandtowpolarityTheresultofMSshowsmolecularweightis352ExaminationontumorcellshowsthesubstancehavestrongantitumoractivityThesubstancehavegreatdevelopingvalueKeyWords：vincaalkaloids；anhydrovinblastine；synthesis；theacti