聚ADP核糖聚合酶(PARP)在人肺腺癌A549細(xì)胞低劑量輻射超敏感性中作用的研究.pdf

1、華中科技大學(xué)碩士學(xué)位論文聚ADP核糖聚合酶（PARP）在人肺腺癌A549細(xì)胞低劑量輻射超敏感性中作用的研究姓名：劉波申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：腫瘤學(xué)指導(dǎo)教師：程晶伍鋼20090501華中科技大學(xué)碩士學(xué)位論文華中科技大學(xué)碩士學(xué)位論文2StudyontherloeofPARPinHRSinhumanlungadenocarcinomaA549cellsCancerCenterUnionHospitalTongjiMedicalCollegeo

2、fHuazhongUniversityofScienceTechnologyWuhan430023ChinaCidate:LiuBoSupervis:Prof.ChengJingProf.WuGangAbstract【Objective】TostudytherelationtotheactivationofPARPradiationdose.ToinvestigatePARPmediatedcelldamagerepairtherela

3、tionshipwiththeHRS.【Methods】StingbyflowanalysisofcolonyfmingcountsofdifferentdosesofcellsurvivalafterirradiationSceobservationcellradiationsensitivitytherelationshipwithradiationdose.Cellsafterirradiationbytrypsindigesti

4、onanalysisoftheuseofcolonyfmingcellsurvivalcurvemappinganalysisofchangesincellsensitivitytoradiotherapy.Electronmicroscopyobservationofcellsinthemicroscopeatdifferentdosesatdifferenttimepointscelldeathunderthecircumstanc

5、esunderstthemodeofcelldeath.WesternblotwithdifferentdosesatthelevelofPARPproteinactivitydrawadifferentdoselevelsofPARPunderthecurvecellsurvivalobservedtherelationshipbetweensces.【Results】⑴A549cellsinbetween0~0.3Gydoseuni

6、tsdemonstratedthephenomenonofantienhancedwiththeincreaseindosebecomessmoothcurveinbetween0.3~0.5Gyradiationshowsomeresistance0.5GyafterregionalcellsurvivalScewiththeincreaseinradiationdosetoreduce.⑵A549cellswereirradiate