解郁膠囊對(duì)抑郁癥大鼠行為學(xué)、腦神經(jīng)遞質(zhì)、血液ACTH、CORT等的影響.pdf

1、甘肅中醫(yī)學(xué)院碩士學(xué)位論文解郁膠囊對(duì)抑郁癥大鼠行為學(xué)、腦神經(jīng)遞質(zhì)、血液ACTH、CT等的影響姓名：馬妮申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：中西醫(yī)結(jié)合臨床指導(dǎo)教師：@201105解郁膠囊對(duì)慢性應(yīng)激抑郁癥大鼠模型的作用和機(jī)理研究2AbstractObjective：ToinvestigatetheeffectsofJieYucapsuleonbehavi、concentrationofDA5HTinBrainNeurotransmitters、conce

2、ntrationofACTHCTinblood、mphologyofhippocampalneuronalganizationinbrainofchronicstressratmodelofdeppressionunderlyingitMethods：72SDrats(SPF)weighting20020gweredividedintosixgroupsbasedongenderweightromly，includingcontrolg

3、roupmodelgroupJieYucapsulelowdosegroupJieYucapsuledosegroupJieYucapsulehighdosagegroupPuYucapsulegroup.Eachgroupwascomposedof6malerats6femalerats.Besidesthecontrolgrouptheratsofothergroupswereinducedtodepressionbychronic

4、stressingf21daysWhilethecontrolgroupmodelgroupweregivenphysiologicsalinebyintragastricadministrationatthe22nddaytheothergroupsweretreatedwithappropriatedosesofJieYucapsulePuYucapsule.Eachdrugwasusedf21daysonceaday.Theeth

5、ologyofratswasobservedtheweightofratsweremeasuredonceaweekduringthisexperiment.AttheendoftheexperimentACTHCTinfemalarterybloodweredetected.RightbrainhippocampalneuronalganizationwasseparatedhomogenatedfDA（dopamine）、5–HT(

6、5hydroxytryptamine)detecting.Theleftbrainhippocampalneuronalganizationwasfixedby4%fmaldehydefhistopathologicexaminationResults：①Comparedwithblankgroup，theweightbehavialindexofchronicstressgroupsweresignificantlychanged.T

7、hisresultsindacatedthatchronicstresscanleadtodepressedmodeinthisexperimentalconditions②JieYucapsulecanincreasetheweightexperimentalsceofopenfieldverticalmovementofrats(P005).Theexperimentalsceoftheopenfieldhizontalmoveme

8、ntwassignificantlyincreasedinJieYucapsulehighdosagegroup(P005).ThesugarwaterconsumptionofexperimentalratsweremarkedincreasedinJieYucapsulehighlowdosesgroups(P005).HighdosesofJieYucapsulereducefcedswimmingfixedtimeoftheex

9、perimentalrats(P005)③HighdledosesofJieYucapsuleincreasedtheconcentrationof5HTDAtodifferentextentsinbrainsoftheexperimentalrats(P005)④HighdledosesofJieYucapsulereducedtheconcentrationofACTHCTtodifferentextentsinthebloodof