哺乳動(dòng)物病毒型TLRs基因的適應(yīng)性進(jìn)化及鯨類(lèi)TLR8的功能驗(yàn)證研究.pdf

1、學(xué)位論文獨(dú)創(chuàng)性聲明本人鄭重聲明：所提交的學(xué)位論文是本人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作和取得的研究成果。本論文中除引文外，所有實(shí)驗(yàn)、數(shù)據(jù)和有關(guān)材料均是真實(shí)的。本論文中除引文和致謝的內(nèi)容外，不包含其他人或其它機(jī)構(gòu)已經(jīng)發(fā)表或撰寫(xiě)過(guò)的研究成果。其他同志對(duì)本研究所做的貢獻(xiàn)均己在論文中作了聲明并表示了謝意。學(xué)位論文作者簽名：岳稍日期：如f6上舛學(xué)位論文使用授權(quán)聲明研究生在校攻讀學(xué)位期間論文工作的知識(shí)產(chǎn)權(quán)單位屬南京師范大學(xué)。學(xué)校有權(quán)保存本學(xué)位論文的電子和

2、紙質(zhì)文檔，可以借閱或上網(wǎng)公布本學(xué)位論文的部分或全部?jī)?nèi)容，可以采用影印、復(fù)印等手段保存、匯編本學(xué)位論文。學(xué)?？梢韵驀?guó)家有關(guān)機(jī)關(guān)或機(jī)構(gòu)送交論文的電子和紙質(zhì)文檔，允許論文被查閱和借閱。(保密論文在解密后遵守此規(guī)定)保密論文注釋?zhuān)罕緦W(xué)位論文屬于保密論文，密級(jí)：——保密期限為——年。學(xué)位論文作者簽名：萄準(zhǔn)鬲指導(dǎo)教師簽名：么名徨淬Et期：00“!2中Et期：oo形‘之_AbstractMammalsarethehighestformofverteb

3、ratesandholdadominantpositionontheearthMostofthemarewithhaitconstanttemperatureandavarietyoffeedinghabitsThelivingenvironmentsofmammalsarevariousincludingterrene，ocean，skyandcaveDuetothevastanddiversityofmammals，somespec

4、ialgroupsofthemareboundtohappensignificantlydifferentadaptivechangeInnateimmunity，asasystemthatmostanimalsuseagainstpathogenicmicroorganisminvasion，isrelativelyoldandthebody’Sfirstlineofdefenseagainstpathogenicmicroorgan

5、ismsTolllikereceptors(TL黜)belongtoanoldfamilywhichmediatesinnateimmunity，dividedintoviralTLRsandnonviralTLRsAmongthem，TLR3，TLR7，TLR8andTLR9belongtoviralTLRsTheyarelocatedonthemembranesofendosomes，andidentifytheviralnucle

6、icacidsBecauseofthegreatdifferencesinlivingenvironmentsandfeedinghabitsofmammals，theviruses’typesarealsodifferentHowevertheresearchontheevolutionmechanismofviralTLRsiSgenerallyconsideredtobeconservativeToexplorethepossib

7、lemolecularmechanismoftheevolutionofmammals’viralTLRs，TLR3，TLR7TLR8andTLR9werechosenforevolutionaryanalysisill27representativemammalsTheselectivetestimplementedwithSiteModel，BranchsiteModelinPAMLandSLAC，F(xiàn)EL，RELillDatamon

8、keydiscoversagreatnumberofpositiveselectedsitesinthefourviralTLRsofmammalsandthemostinTLR9MostoftheSepositiveselectedsitesarelocatedintheLRRdomainintheextracellulardomainLRRdomainistheregionwhichdirectcombinewithPAMPs，an

9、dhasbeenunderstrongpositiveselectionThroughtheanalysisoftheBranchsiteModelandfleeratiomodel，itisshowedthatviralTLRshasbeenunderstrongpositiveselectedpressuresiIlcetaceansandChiropteraThisindicatesthatthetypesandnumberofv

10、irusesofcetaceansandChiropterahavechangedduetothemarineenvironmentandtheskycavesenvironmentiIlthelongprocessofevolutionandtheselectedpressureoftheirviralTLRshaschangedtooWealsofindpositiveselectedsignalsintheotherbranche

11、ssuchasCetartiodactylaandPrimatesItisshowedthemammalianviralTLRshavebeenunderuniversalpositiveselectionWedetectedthemostcommonpositiveselectedsignalsinTLR9，suggestingthatitmaybetheonlyTLRtoidentifytheviralDNAThismayberes