杠柳根皮化學(xué)成分及生物活性的研究.pdf

1、Y833856分類號：Q94683UDc：81：塑密級：公開級學(xué)校代碼110712研究生學(xué)號：TG02178西弛農(nóng)林科教大學(xué)、。2005屆在職攻讀碩士學(xué)位研究生學(xué)位論文杠柳根皮化學(xué)成分及生物活性的研究類型：農(nóng)業(yè)推廣領(lǐng)域、專業(yè)、方向：種植研究生：史清華指導(dǎo)完成教師：時(shí)間：馬養(yǎng)民副教授二00五年十月中國陜西楊凌ABSTRACTASTUDYONCHEMICALCOMPOSITIONANDBIOACTIVITYOFRoOTBARKFROM只E&

2、滬己D(■S!E阿(刀MABSTRACTPeriplocasepeiumBgebelongstoAsclepiadaceaefamilyanddistributeswidelyinChiIlaInshaanxi’province，theplantchieflygrowthsinQin—Bamountain，Guanshanmountain，HuanglongmountainandQiaoshanmountainandhasplentyo

3、fresourceInorder，toexploitandusetheplantresource，westudiedthechemicalcompositionandfarmfunction●ontherootbarkofPeriplocasepiumApreliminarystudyonchemicalcompositionofrootbarkfromPeriplocasepiumWasaccomplished。ItiSindicat

4、edthattherootbarkcontainsaminoacidscarbohydrateanditsglycosides，organicacids，saponins，alkaloid，phenols，cardiacglycosides，lactones，coumarinsanditsglycosides，sterols，triterpenes，volatileoil，fatsAlkaloidofthemiSfirstdetermi

5、ned。intheplantMeanwhile，Extractsofpetroleum，ethylacetate，n—butylalcoholandwaterfrommethanolcrudeextractsoftherootbarkwereusedtotesttheinsecticidalactivityagainstSchizaphisgraminum(Rond)andMythimnaseparata(Walker)Theresul

6、tsshowedthatnbutylalcoh01extractspossessobviouscontactpoisonagainstSchizaphisgraminum(Rond)andcertainstomachpoisonagainstMythimnaseparata(Walker)，ThechemicalcompositionofthevolatileoilfromPeriplocasepiumrootbarkwasanalyz

7、edbymeansofgaschromatography—massspectrometer(GC／MS)technique10compoundsof15componentsseparatedfromthevolatileoilwhichconstitute9491％thetotaloil，wereidentifiedtheyare4methoxysalicytaldehyde，butylacetate，2methyl—l，3一dioxy

8、—cyclopentyl—ethylacetate，S[8一(diethylphosphono)octyl】ethanethioate，1，1，3，3，一tetrabutoxy一2一propanone，4methyl一2pentanone，butylfonrtate，1一(1ethoxyethoxy)一butane，2butoxytetrahydropyran，butyletherMeanwhile，theresultoftestoni

9、nsecticidalactivityofthevolatileoilaccomplishedshowedthattheoilpossessesobviouscontactpoisonagainstSchizaphisgraminumFourextractsofpetroleumether，ethylacetaten—butan01andwaterwereobtainedfromthemethanolextractoftherootba

10、rkinPeriploeasepeiumCompoundIandcompoundsII，IIIIVwereisolatedrespectivelyfrometheracetateextractandn—butanolextractbycolumnchromatographyofsilicagel，C18andSephadex—LH一20ThefourcompoundswereidentifiedasB—amyrinacetate，4me

11、thoxysalicylaldehydelactoside，periplocosideEandacardiacglycosidewithchemicalmethodandspectralanalysis(IR，。H剞MR，”CNMR，El—MS)Meanwhile，compoundsII，Ili，IVwereusedtotestOUbioactivityTheresultsshowedthatIIrestrainwheatandrape