淡水噬藻體若干生理生態(tài)學特性的研究.pdf

1、華中師范大學碩士學位論文淡水噬藻體若干生理生態(tài)學特性的研究姓名：程凱申請學位級別：碩士專業(yè)：微生物指導教師：趙以軍2002.5.1AbstractInvestigationindetailsonafreshwatercyanophageisolatedinChinainfectingPlectonemaandPhormidiumispresentedinthisthesisUnderTrasmissionElectronicMicros

2、cope(TEM)，thecyanophageshowedabacteriaphageshapewithheaddiameterat52nmandshorttail(almostinvisible)SeveralmethodsformeasuringthelysingcycleandburstsizeofthecyanophageweretestedItwasestablishedthatcellcountingmethodwasmor

3、econvenientandexactforconfirmingthelysingcycleBurstsizeobtainedbylPFUinfectingwasreliableMorestudieswereconductedOilboththephysiologyandecologyofthecyanophageResultsinvolvedinthephysiologicalcharactersareasbelow：thecycle

4、oflysingPlectonemaat28℃was4hrsincluding3hrsofpatentperiodandlhroflysingperiodTheburstsizeofthecyanophagewasca200PFU‘Cell～TherateofadsorptiontOhostWaSmarkedlyhigherunderlightconditionthanthatunderdarkcondition(P2002)，andl

5、ightWaSnecessaryforinfectionMoreovertheadsorptionrateWasaffectedbythegrowthofhost：itbecamegreateralongwiththehostgrowthandreachedthemaxiumatstablestageDuringinfection，thephotosyntheticoxygenreleasingratedecreaseddrastica

6、llyTheactivityofcyanophagechangedunderdifferenttemperatures：100％activityremainedat40“Cfor10mio，morethan75％at50“Cforlmin，60％at50“Cfor10min，15％at60℃forlminandlessthan1％at60℃for】0minItWasalsoindicatedthattheenlargementofpla

7、querelatedtothegrowthofhost，ie，themorerapidthegrowth，themorenotabletheenlargementoftheplaquePreliminarystudiesontheecologicalcharactersoffreshwatercyanophagewerecarriedoutunderbothfieldconditionandculturingsystemThefield

8、investigationresultshowedthat6in19watersamplescontainedcyanophageslysingplectonemaandthelysisphenomenonwerereadilyobservedwithoutconcentratingthewaterThusitcouldbepresumedthatthereWasawidespreaddistributionandahighinfect