植物與微生物細(xì)胞壁糖的熒光輔助糖電泳分析.pdf

1、1J005057r，、#JtI℃辜卞斜筱大浮碩士學(xué)位論文植物與微生物細(xì)胞壁糖的熒光輔助糖電泳分析’；’化111L’J人‘利0qk卅甘投川J柞卅ff1[I】∞、唬；小物J，、學(xué)【m‘劉世曲救投20()iIr【】Jl2華中科技大學(xué)碩士學(xué)位論文AbstractSaccharidesaremajorstructureandfuctionalcompositionsoflivingthingsEspeciallyoligosaccharidesa

2、remajorparticipatorsinthelivingprocessSomeactiveoligosaccharidesarefromcellwallsofplantsandmicrobesToinvestigateoligosaccharides，weextractcellwallpolysaccharidesfromcellwallofSaccharomycescerevisiaeanddrygrazingbyalkalin

3、e—hydrolyzing，extractoligoxylanfromalkalinehydrolyzedgrazingbycrystaling，extractwatersolubilityextracellularpolysaccharidesbetweenkelpcellsbywaterdistilling，extractcellwallpolysaccharidesofSaccharomycescerevisiaeandwater

4、solubilityextracellularpolysaccharidcsfromkelpcellshydrolysatebyacid—hydrolyzingThenweanalyzethembythewayoffluorophore—assistedcarbohydrateelectrclphoresisSubsequentlNwecomparetheresultswithelectrophoreticchanoftheoligos

5、accharideadmixturefromacidhydrolyzedwatersolubilitystarchFluorophore—assistedcarhohs’drateelectrophoresis(FACE)isasimpleandinexpensivemethodforseparatingthecarbohydratesThe(01培o)saccharidesweretaggedwiththechargedfluorop

6、hore8aminonapbthalene一】，3，6一trisul^onate(ANTS)，andthereductiveaminationreactionswereessentiallycompleteafterapproximately16hrunderthegivenexperimentalConditionsSaccharide—ANTSadductswerethenseparatedfromoneanotherbyelect

7、rophoresisona32％CACR24％CBISpolyacrylamidegelatalkalinepHThistechniquedoesn’trequiresophisticatedinstrumentationandhighlytrainedpersonnelTheexperimentalresultsindicate：1，Theoligosaceharideadmixturefromacidhydrolyzedwaters

8、olubilitystarchCallformaseriesofbondsinelectrophoreticchanAndthefirstandsecondbondsarematchedtoglucoseandmaltose，separatelyItindicatesthatelectrophoreticchartoftheh，’dmlysateofwatersolubilitystarchcanshowthegradsdistribu