高效微生物絮凝劑產(chǎn)生菌的選育及應(yīng)用研究.pdf

1、沈陽建筑大學(xué)碩士學(xué)位論文高效微生物絮凝劑產(chǎn)生菌的選育及應(yīng)用研究姓名：霍石磊申請學(xué)位級別：碩士專業(yè)：環(huán)境工程指導(dǎo)教師：魏煒201101碩士研究生學(xué)位論文AbstractIIIAbstractSewagewaterqualitydegradationoftheincreasinglycomplexmedifficulttosubstancesintheemergenceofthetraditionalwatertreatmentagenth

2、asbeendifficulttomeettherequirementsofmodernsewagetreatment.Flocculantinsewagetreatmentintheapplicationoftherelationshipjuiceathomeabroad，mainlyintheflocculatingactivityofmicrobialscreeningwk.Flocculantfsewagetreatmentin

3、theprocessingcostisrelativelyhigh，resultingflocculantisdifficulttoachieveawiderangeofindustrialapplications，reducetheircostsofdealingwithless.Thisarticleasastartingpoint，thesewagetreatmentplantbyactivatedsludgeseparation

4、，ionofefficientstrainsofmicrobialflocculantactivity，reducetheproductioncostofmicrobialflocculantsexperimentalstudycarriedout.FromShenyangCity，nthofactivatedsludgesewagetreatmentplantseparatedby96breedingmicroganisms，whic

5、hhastheflocculatingactivityofa30microganisms，Thenbyexpingcultivationmethodskaolinflocculationtest，getthebestflocculatingactivityofastrainJB5，ObtainedbyUVmutagenesisofstrainJB5mutantstrainJB5B，itsintheflocculationofkaolin

6、suspensiontoreachthehighestrateof95.7%.ByGramstaining，electronmicroscopy，theirphysiologicalbiochemicalacteristics，derivedstrainsJB5BJB5BftheGramnegativebacteria，bothfshtrod，oxidasetestwasnegative，starchhydrolysisintothen

7、egative，thatstarchhydrolysisisnotstrong；Methylredtestpositivestaining，indicatingthatglucosefermentationcapacityofstrongacid.Flocculantfbyanthronetest，biurettestninhydrintestshowsthattheresultingstrainscontainpolysacidepr

8、oteinflocculantmacromolecules.FlocculationtoKaolinsuspensionastheobject，JB5Bproducingflocculantfoptimizationofcultureconditions，strainJB5Bofthebestconditionsfflocculantproduction：glucose40gL，KNO31gL，K2HPO45gL，KH2PO42gL，M

9、gSO40.1gL，NaCl0.1gL，carbonnitrogenratioof50，carbonconcentrationof45gL，initialpH8.0，culturetemperature30℃，vaccinationvolumeof3mL，160rminshakingculture30h.Undertheseconditions，thehighestflocculatingrateofupto95.7%.Theappli

10、cationoftheabovetestconditionsfflocculant，carrierKaolinsuspensionoftheeffectsofflocculation，pHvalueswerediscussed，Ca2solutiondosage，theamountofbacterialsuspensiondosageeffectontheflocculation，thethogonaltest.Strainderive

11、dfromthefermentationbrothof4gLofkaolinsuspensionwasthebestflocculationconditions：pHvalueof8，add1%Ca2solutiondosageof3.5mL，theamountofdosageofbacilli3mL，intheconditionsKaolinsuspensionunderitsflocculationrateof95.7%.obtai