簡(jiǎn)介：SINGLEMOLECULEBIOPHYSICS,,,23294,SINGLEMOLECULE,,ASOF2003SINGLEMOLECULESCEASEDPUBLICATIONANDTHEARTICLESAREINCORPORATEDINCHEMPHYSCHEM,LESS,FASTER,IMPETUSTOSINGLEMOLECULE,WHATISSINGLEMOLECULEBIOPHYSICS,WHYWECONDUCTSINGLEMOLECULEORWHATSINGLEMOLECULECANTELLUS,HOWTOSTUDYTHESINGLEMOLECULES,,SINGLEMOLECULESINDIVERSEENVIRONMENTSSUCHASLIQUIDS,SOLIDS,MEMBRANES,SURFACES,ANDPOLYMERS,SINGLEMOLECULESPROVIDESABROADOVERVIEWOFCURRENTMETHODSANDTECHNIQUES,RECENTAPPLICATIONSANDSHORTCOMINGSOFPRESENTTECHNIQUESINTHEFIELDITCOVERSALLASPECTSOFSINGLEMOLECULEINVESTIGATIONSINCHEMISTRY,PHYSICS,ANDLIFESCIENCES,METHODSOFMANIPULATINGANDMODIFYINGSINGLEPARTICLES,SINGLEATOMSANDNANOPARTICLES,ALLKINDSOFULTRASENSITIVEDETECTIONTECHNIQUESSUCHASSPECTROSCOPIESANDMICROSCOPIES,WHATISSINGLEMOLECULEBIOPHYSICS,在單分子水平上對(duì)生物分子行為（包括構(gòu)象變化、相互作用、相互識(shí)別等）的實(shí)時(shí)﹑動(dòng)態(tài)檢測(cè)以及在此基礎(chǔ)上的操縱﹑調(diào)控等，是分子生物物理學(xué)的自然延伸和必然趨勢(shì)。生物單分子研究已成為21世紀(jì)生命科學(xué)領(lǐng)域的一個(gè)重點(diǎn)研究方向，在生命科學(xué)領(lǐng)域有著廣泛的應(yīng)用前景,WHYWECONDUCTSINGLEMOLECULEORWHATSINGLEMOLECULECANTELLUS,INCONTRASTWITHENSEMBLEMETHODS,SINGLEMOLECULEEXPERIMENTSPROVIDEINFORMATIONONDISTRIBUTIONSANDTIMETRAJECTORIESOFOBSERVABLESTHATWOULDOTHERWISEBEHIDDEN,SINGLEMOLECULEMETHODS,SENSITIVEORULTRASENSITIVE,,SENSITIVITY,,THEPHOTONSDETECTEDFROMSINGLEMOLECULESMUSTEXCEEDTHEBACKGROUNDNOISETHATORIGINATESFROMRAMANANDRAYLEIGHSCATTERINGANDFROMFLUORESCENCEASARESULTOFIMPURITIESINTHESOLVENT,GLASSCOVERSLIPS,ANDOPTICALCOMPONENTS,ANDFROMDARKCURRENTINTHEDETECTOR,GENESISOFNOISE,ASMALLEXCITATIONVOLUME,TOREDUCETHEBACKGROUND,HIGHEFFICIENCYCOLLECTIONOPTICS,DETECTORSWITHHIGHQUANTUMEFFICIENCYANDLOWDARKNOISE,CAREFULELIMINATIONOFBACKGROUNDFLUORESCENCESOLVENT,OPTICS,DETECTIONMETHODETAL,HOWTOINCREASES/N,TECHNIQUESTOSTUDYTHESINGLEMOLECULE,LASERSCANNINGCONFOCALMICROSCOPYLSCM,TOTALINTERNALREFLECTIONFLUORESCENCEMICROSCOPYTIRFM,NEARFIELDSCANNINGOPTICALMICROSCOPYNSOMORSNOM,SCANNINGPROBEMICROSCOPYSPM,SCANNINGTUNNELINGMICROSCOPESTM,ATOMFORCEMICROSCOPEAFM,OPTICALTWEEZER/OPTICALTRAPOPOROT,,ASINGLEMOLECULESTUDYOFRNACATALYSISANDFOLDING,THENOBELPRIZEINPHYSICS1997“FORDEVELOPMENTOFMETHODSTOCOOLANDTRAPATOMSWITHLASERLIGHT“,STEVENCHUUSA,CLAUDECOHENTANNOUDJIFRANCE,WILLIAMDPHILLIPSUSA,在獲得諾貝爾獎(jiǎng)的第二天，他騎著自行車(chē)，朝著目標(biāo)往山路上攀爬，達(dá)到了目的地。這種攀登高峰的踏實(shí)感受也只有在努力過(guò)之后，才能真切地感受到,光束密糖?MOLASSES,朱棣文江蘇太倉(cāng),ASINGLEMOLECULESTUDYOFRNACATALYSISANDFOLDING,XIAOWEIZHUANG,LAURAEBARTLEY,HAZENPBABCOCK,RICKRUSSELL,TAEKJIPHA,DANIELHERSCHLAG,STEVENCHU1SCIENCE,2000,288,20482051,,HOWARDHUGHESMEDICALINSTITUTEINVESTIGATORPROFESSOROFCHEMISTRYANDCHEMICALBIOLOGYPROFESSOROFPHYSICS,FRETTIMETRACESHOWINGUNFOLDINGANDREFOLDINGOFARIBOZYMEMOLECULE,05S,INTERMEDIATE,,ASINGLEMOLECULESTUDYOFPROTEINFOLDING,,,,FOLDINGIMAGEOFSINGLEGCN4DIMERS,,UREA,UREA,,DONORR6G,ACCEPTORTXR,SINGLEGCN4DIMERSAT0MUREAPH61,SINGLEGCN4DIMERSAT74MUREAPH61,STRONGACCEPTOREFFICIENTFRETFOLDED,STRONGDONORXIAO,JIEREN,XIAOJIALAO,KAIQINXIE,XSUNNEYSCIENCE,311,160016032006,STOCHASTICPROTEINEXPRESSIONININDIVIDUALCELLSATTHESINGLEMOLECULELEVEL,CAI,LONGFRIEDMAN,NIRXIE,XSUNNEYNATURE,440,3583622006,PROBINGGENEEXPRESSIONINLIVECELLS,ONEPROTEINMOLECULEATATIME,,TSRVENUS,TRANSCRIPTIONOFONEMRNABYANRNAPOLYMERASERESULTSFROMANINFREQUENTANDTRANSIENTDISSOCIATIONEVENTOFREPRESSORFROMDNA,SCHEMEOFLIVECELLOBSERVATIONSOFGENEEXPRESSION,SINGLEMOLECULEDETECTIONOFAFLUORESCENTFUSIONPROTEIN,TSRVENUS,INLIVEECOLICELLS,,REALTIMEMONITORINGOFTHEEXPRESSIONOFTSRVENUSUNDERTHECONTROLOFREPRESSEDLACPROMOTER,,USINGSINGLEMOLECULETECHNOLOGYTOSTUDYTHEINFECTIOUSPATHWAYOFVIRUS,,,,REALTIMESINGLEMOLECULEIMAGINGOFTHEINFECTIONPATHWAYOFANADENOASSOCIATEDVIRUS,SEISENBERGERGETALSCIENCE,2001,294192932,病毒AAV細(xì)胞HELA熒光探劑CY5CARBOCYANINEDYE,1/25儀器CCD（電荷耦合探測(cè)器）測(cè)量74個(gè)細(xì)胞，1009AAVCY5軌跡，,病毒通過(guò)細(xì)胞表面時(shí)會(huì)慢下來(lái)。然后病毒接觸細(xì)胞表面好幾次，多數(shù)情況下沒(méi)有應(yīng)答，只有13的病毒通過(guò)細(xì)胞表面,大約三分之一的病毒顆粒延規(guī)定好的途徑定向移動(dòng),一旦其進(jìn)入細(xì)胞，病毒有50的幾率達(dá)到細(xì)胞核,DNAIMAGETAKENBYEVANESCENTMICROSCOPESINGLEMOLECULEOBSERVATION,LOWBACKGROUND,,BYAFM,PROGRESSINBIOPHYSICSFRIEDMAN,NIRXIE,XSUNNEY,STOCHASTICPROTEINEXPRESSIONININDIVIDUALCELLSATTHESINGLEMOLECULELEVEL,NATURE,2006,440,358362,
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